PUBLICATIONS - W. Scott Champney

W. Scott Champney, Professor. (champney@etsu.edu) Ph.D. SUNY at Buffalo. macrolids antibiotic effects on bacterial ribosome structure and function. Genetic analysis of ribosomal protein function.

Research summary statement:

We are studying the novel inhibitory effects of macrolide and ketolide antibiotics on bacterial ribosome formation. 50S ribosomal subunit formation in cells is specifically prevented and this activity is equivalent to translation as a target for drug inhibition of growth in bacterial cells by several different macrolide compounds.  We have examined over 25 different macrolide and ketolide componds for this hibitory activity in an investigation of structure-activity relationships. Our findings can be exploited to develop novel antimicrobial compounds which will specifically attack this new target and which may be more effective than current antibiotics against bacterial infections. Much of our work has focused on the organism Staphylococcus aureus,  an important human pathogen. Our research has shown that antibiotic-resistant Staphylococcus aureus cells can be killed by some of these antibiotics, thus offering a new weapon in the fight against drug-resistant bacteria.

Our future research plans include an examination of the effects of macrolide and ketolide antibiotics on ribosomal function and assembly in Streptococcus pneumoniae, a human respiratory pathogen. We are also examining a number of other antimicrobial agents for their effects on translation and ribosome biogenesis. We have  planned molecular investigations of the mechanism of subunit assembly inhibition in growing cells.

Mehta, R, Champney, W.S.  2002.  30S Ribosomal subunit assembly is a target for inhibition by aminoglycoside antibiotics in Escherichio coli. Antimicrobial Agents Chemother.  46:1546-1549.

Champney, W.S., Miller, M.  2002.  Linezolid is a specific inhibitor of 50S ribosomal subunit formation in Staphylococcus aureus cells. Current Microbiology 44(5):350-356.

Champney, W.S., Miller, M.  2002.  Inhibition of 50S ribosomal subunit assembly in Haemophilus influenzae cells by azithromycin and erythromycin. Current Microbiology   44(6): 418-424

Champney, W.S., Pelt, J.  2002. The ketolide antibiotic ABT-773 is a specific inhibitor of translation and 50S ribosomal subunit formation in Streptococcus pneumoniae cells. Current Microbiology  45: 155-160

Champney, W.S., Pelt, J.  2002. Telithromycin inhibition of protein synthesis and 50S ribosomal subunit formation in Streptococcus pneumoniae cells. Current Microbiology  45: 328-333.

Champney, W.S.  2001.  Bacterial ribosomal subunit synthesis: a novel antibiotic target. Current Drug Targets - Infectious Disorders  1: 19-36

Champney, W.S., Pelt, J., Tober, C.L.  2001.  TAN 1057A: a translational inhibitor of 50S ribosomal subunit activity and formation. Current Microbiology  43: 340-345

Champney, W.S., Tober, C.L.  2001.  Structure-activity relationships for six ketolide antibiotics. Current Microbiology  42: 203-210

Usary, J., Champney, W.S.  2001.  Erythromycin inhibition of 50S ribosomal subunit formation in Escherichia coli cells. Molec. Microbiol.  40: 951-962

Champney, W.S., Tober, C.L.  2000.  Specific inhibition of 50S ribosomal subunit formation  in Staphylococcus aureus  cells by 16-membered macrolide, lincosamide and streptogramin B antibiotics.  Current Microbiology  41: 126-135.

Champney, W.S., Tober, C.L.  1999.   A molecular investigation of the post-antibiotic effect of clarithromycin and erythromycin on Staphylococcus aureus  cells. Antimicrobial Agents Chemother. 43: 1324-1333.

Champney, W.S., Tober, C.L.  1999.  Superiority of 11,12 carbonate macrolide antibiotics as inhibitors of translation and 50S subunit formation inStaphylococcus aureus  cells. Current Microbiology 38: 342-348.

Lingerfelt, B., Champney, W.S.  1999.  Macrolide and ketolide antibiotic separation by reversed phase high performance liquid chromatography. J. Pharmaceut. and Biomed. Analysis  20: 459-469

Champney, W.S.  1999.  Macrolide antibiotic inhibition of 50S ribosomal subunit formation in bacterial cells. Recent Research Developments in Antimicrobial Agents and Chemotherapy  3: 39-58.

Champney, W.S. & C. L. Tober  1999.  Evernimicin (SCH 27899) inhibits both  translation and 50S ribosomal subunit formation in Staphylococcus aureus cells.  Antimicrobial Agents Chemother.  44: 1413-1417.