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Biological Sciences

College of Arts & Sciences

Cecilia A. McIntosh

Cecilia A. McIntosh 

Professor
Contact:
423-439-5838 /
Brown Hall, Room 428

Research Description:

Our overall research program related to plant secondary metabolism, specifically flavonoid biosynthesis and regulation.  We have isolated a flavonol-specific glucosyltransferase from grapefruit which possesses unique substrate and regiospecificity.  We have used bioinformatics, sequence alignments, 3-D homology modeling, and site-directed mutagenesis to test hypotheses related to amino acids critical for the structure and function of this enzyme. Current efforts involve preparation of the wild type enzyme and three mutants for protein crystallization the results of which will further test our homology models and hypotheses.

Representative recent publications (* indicates student co-authors)

Owens, Daniel K. and Cecilia A. McIntosh (2011) “Biosynthesis and Function of Citrus Glycosylated Flavonoids”, Recent Advances in Phytochemistry, vol. 41:67-95 (Invited, Peer-reviewed).

Daniel*, J.J., D.K. Owens, and C.A. McIntosh.  (2011).  Secondary Product Glucosyltransferase Expression During Citrus paradisi Growth and Development. Phytochemistry 72:1732-1738.

Jackson*, A.R., D. Knisley, C. McIntosh, and P. Pfeiffer. (2011). Predicting Flavonoid UGT Regioselectivity.  Advances in Bioinformatics  2011( ID 506583): 15 pp. (doi:10.1155/2011/506583)

McIntosh, C.A., K. Bartoszuk, and B.J. Maxson.  (2014) Integration and Synergy of Research and Graduate Education in Science, Humanities, and Social Sciences.  Critical Conversations: An Interdisciplinary Journal 1:70-92. (invited, peer-reviewed)

McIntosh, Cecilia A. and Daniel K. Owens. (2016). Advances in Flavonoid Glycosyltransferase Research: Integrating Recent Findings with Long-Term Citrus Studies.  Phytochemistry Reviews 15:1075-1091. doi 10.1007/s11101-016-9460-6

Devah, S.K., D.K. Owens, M.B. Sibhatu*, T. Roy Sarkar*, C.L. Strong*, V.K.P.S. Mallampalli*, J. Asiago*, J. Cooke*, S. Kiser*, Z. Lin*, A. Wamucho*, D. Hayford*, B.E. Williams*, P. Loftis*, M. Berhow, L.M. Pike. and C.A. McIntosh.  (2016).  Isolation, Recombinant Expression and Biochemical Analysis of Putative Secondary Product Glucosyltransferases from Citrus paradisi.  J. Agric. Food. Chem. 64:1957-1969 (doi 10.1021/acs.jafc.5b05430)

Devaiah, S.P., B.M. Tolliver*, C. Zhang*, D.K. Owens, and C.A. McIntosh. (2017). Effect of Amino Acid Insertion and Other Mutations on the Substrate and Regiospecificity of a Citrus paradisi Flavonol-Specific 3-O-Glucosyltransferase. J Plant Biochem and Biotech. IN PRESS DOI: 10.1007/s13562-017-0411-0

RoySarkar, T., C.L. Strong, M.B. Sibhatu, L.M. Pike, and C.A. McIntosh. 2007. Cloning, Expression, and Characterization of a Putative Flavonoid Glucosyltransferase from Grapefruit (Citrus paradisi) Leaves. Chapter 18 in “Concepts in Plant Metabolomics”, B.J. Nikolau and E.S. Wurtele, eds. Springer Publishing, The Netherlands. pp. 247-259 (Invited submission).

Knisley, D., E. Seier, D. Lamb. D.K. Owens, and C.A. McIntosh. 2009. A Graph-theoretic Model Based on Primary and Predicted Secondary Structures Reveals Functional Specificity in a set of Plant Secondary Product UDP-Glucosyl-transferases. Proceedings of the 2009 International Conference on Bioinformatics, Computational Biology, Genomics, and Chemoinformatics, W. Logong, M. Doble, Z. Sun, and J. Malone (eds), pp 65-72. (Peer-reviewed, Invited)

Owens, D.K. and C.A. McIntosh. 2009. Identification, recombinant expression, and Biochemical Characterization of a Flavonol 3-O-Glucosyltransferase Clone from Citrus paradisi. Phytochemistry, 70:1382-1391.

Daniel, J.J., D.K. Owens, and C.A. McIntosh. 2011. Secondary product glucosyltransferase expression during Citrus paradisi growth and development. Phytochemistry, 72:1732-1738.

Jackson, A.R., D. Knisley, C. McIntosh, and P. Pfeiffer. 2011. Predicting flavonoid UGT regioselectivity. Advances in Bioinformatics 2011( ID 506583):15 pp. (doi:10.1155/2011/506583).

Owens, D.K. and C.A. McIntosh. 2011. Biosynthesis and function of Citrus glycosylated flavonoids, Recent Advances in Phytochemistry, 41:67-95.

Courses:

  • Teaching Pedagogy for the Graduate Teaching Assistant (GRAD5110)
  • Mentoring Student Research (GRAD7850)
  • Research in Biology (BIOL4910/5910)
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