My research group focuses on chemical mechanisms of oxidative damage to DNA under
conditions mimicking oxidative stress. Free radical damage to DNA by reactive oxygen
species (ROS) overproduced in living organisms due to the effects of ionizing radiation,
UV-light, tobacco smoking, and infections has been unequivocally linked to a number
of debilitating conditions as cancer, cardiovascular and neurodegenerative diseases,
and aging. Therefore, cures for these diseases rely on a better understanding of underlying
mechanisms of DNA damage. The aim of our research is to deepen the understanding of
chemical mechanisms of reactions of ROS with DNA essential for prediction of long-term
biological consequences of these processes and for future elaboration of preventive
measures aimed at controlling DNA damage by free radicals.
Our research group has thoroughly elaborated a set of HPLC-based techniques of quantitative
analysis of low-molecular characteristic products of DNA oxidative damage as a well-established,
powerful, and convenient method of studying mechanisms of oxidative damage to DNA.
Roginskaya, M., Janson, H., Seneviratne, D. Razskazovskiy, Y. The reactivity of 2,5-
diaminoimidazolone base modification towards primary amines: nucleophilic substitution
at C5 as a potential source of abasic sites in oxidatively damaged DNA. Res. Chem.
Intermed. Online publication (2016). Full text is available at http://rdcu.be/kbVS.
Roginskaya, M., Mohseni, R., Ampadu-Boateng, D., Razskazovskiy, Y. DNA damage by the
sulfate radical anion: hydrogen abstraction from the sugar moiety versus one-electron
oxidation of guanine. Free Radic. Res. 50:756-766 (2016).
Roginskaya, M., Moore, T. J., Ampadu-Boateng, D., Razskazovskiy, Y. Efficacy and site
specificity of hydrogen abstraction from DNA 2-deoxyribose by carbonate radicals.
Free Radic. Res. 49:1431-1437 (2015).
Roginskaya, M. Mohseni, R., Moore, T. J. Bernhard, W. A. and Razskazovskiy, Y. Identification
of the C4´-oxidized abasic site as the most abundant 2-deoxyribose lesion in radiation-damaged
DNA using a novel HPLC-based approach. Radiat. Res.181: 131-137 (2014).
Roginskaya, M., Razskazovskiy, Y. Selective radiation-induced generation of 2-deoxyribonolactone
lesions in DNA mediated by aromatic iodonium derivatives. Radiat Res. 171, 342-348
Roginskaya, M., Bernhard W.A, Razskazovskiy, Y. Protection of DNA against direct radiation
damage by complex formation with positively charged polypeptides. Radiat Res. 166,
Roginskaya, M., Bernhard, W. A., Razskazovskiy, Y. 2-Deoxyribonolactone lesions in
X-irradiated DNA: Quantitative determination by catalytic 5-methylene-2-furanone release.
Ang. Chem. Int. Ed. 44, 6210-6213 (2005).
Roginskaya, M., Bernhard, W. A., Marion, R. T., Razskazovskiy, Y. The release of 5-methylene-2-furanone
from irradiated DNA catalyzed by cationic polyamines and divalent metal cations. Radiat.
Res. 163, 79-84 (2005).
Roginskaya, M., Bernhard, W. A., Razskazovskiy, Y. Diffusion approach to long distance
charge migration in DNA: time-dependent and steady-state analytical solutions for
the product yields. J. Phys. Chem. B. 108, 2432-2437 (2004).
Razskazovskiy, Y., Roginskaya, M., Jacobs, A., Sevilla, M. D. Reductively activated
cleavage of DNA mediated by o,o'-diphenylenehalonium compounds. Radiat. Res. 154,
Razskazovskiy, Y., Roginskaya, M., Sevilla, M. D. Modification of the reductive pathway
in gamma-irradiated DNA by electron scavengers: targeting the sugar-phosphate backbone.
Radiat. Res. 149, 422-432 (1998).