Dr. Scott Champney

champney

 

 

 

 

Scott Champney, Ph.D.
Professor, Department of Biomedical Sciences


Contact Information:

Department of Biomedical Sciences
James H. Quillen College of Medicine
PO Box 70582
Room B025 Stanton Gerber Hall (Bldg. 178)
Phone:  423-439-2022
Home: 423-928-1956
Fax:  423-439-2030
Email: 

 

Education:

  • 1965 A.B., University of Rochester
  • 1970 Ph.D., State University of New York at Buffalo (includes 1.5 years of graduate studies in the Deparment of Microbiology, Baylor College of Medicine)

Research/Teaching Interests:

1.  Ribosomes
2.  Antibiotics
3.  Pathogenic Microorganisms


Research Projects:


My research interests have focused on the structural and functional relationships of the different macromolecules in the bacterial ribosome. In particular we have examined the roles of the numerous ribosomal proteins in the complex process of translation. Genetic and biochemical methods have been used to help uncover mutational changes in temperature-sensitive mutants responsible for alterations in protein synthesis. This research has identified specific functional defects in translation associated with mutational changes in single ribosomal proteins. Genes from the mutant cells have been cloned and the sequence alterations defined for several strains. We are also studying the inhibitory effects of antibiotics on bacterial ribosome biosynthesis. Subunit formation in cells is specifically prevented by many different antibiotics and this activity is equivalent to translation as a target for drug inhibition of growth in bacterial cells. This is a new target for antimicrobial agents. This work will help in the development of novel antimicrobial compounds which will specifically attack this new target and which may be more effective than current antibiotics against bacterial infections. We are examining a number of different antimicrobial agents for effects on translation and ribosome biogenesis. We are also currently investigating the mechanism of subunit assembly inhibition by antibiotics.  Research on inhibition of bacterial ribosome biogenesis by antimicrobial agents.

 


 

Lab Members

Mr. Justin Beach, graduate student
 


 Selected References:

1:

A crisis is developing in the treatment of infectious diseases. Preface.

 

Champney WS.
Methods Mol Med. 2008;142:v-vi. No abstract available.
PMID: 18444295 [PubMed - indexed for MEDLINE]
Related Articles

2:

Three methods to assay inhibitors of ribosomal subunit assembly.

 

Champney WS.
Methods Mol Med. 2008;142:63-73.
PMID: 18437306 [PubMed - indexed for MEDLINE]
Related Articles

3:

Characteristics of a 50S ribosomal subunit precursor particle as a substrate for ermE methyltransferase activity and erythromycin binding in Staphylococcus aureus.

 

Pokkunuri I, Champney WS.
RNA Biol. 2007 Nov;4(3):147-53. Epub 2007 Nov 25.
PMID: 18094627 [PubMed - indexed for MEDLINE]
Related Articles

4:

Characterization of a 30S ribosomal subunit assembly intermediate found in Escherichia coli cells growing with neomycin or paromomycin.

 

Foster C, Champney WS.
Arch Microbiol. 2008 May;189(5):441-9. Epub 2007 Dec 5.
PMID: 18060665 [PubMed - indexed for MEDLINE]
Related Articles

5:

Retapamulin inhibition of translation and 50S ribosomal subunit formation in Staphylococcus aureus cells.

 

Champney WS, Rodgers WK.
Antimicrob Agents Chemother. 2007 Sep;51(9):3385-7. Epub 2007 Jun 11.
PMID: 17562806 [PubMed - indexed for MEDLINE]
Related Articles Free article in PMC | at journal site

6:

The other target for ribosomal antibiotics: inhibition of bacterial ribosomal subunit formation.

 

Champney WS.
Infect Disord Drug Targets. 2006 Dec;6(4):377-90. Review.
PMID: 17168803 [PubMed - indexed for MEDLINE]
Related Articles

7:

Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli.

 

McGaha SM, Champney WS.
Antimicrob Agents Chemother. 2007 Feb;51(2):591-6. Epub 2006 Oct 16.
PMID: 17043113 [PubMed - indexed for MEDLINE]
Related Articles Free article in PMC | at journal site

8:

A comparison of a new oral streptogramin XRP 2868 with quinupristin-dalfopristin against antibiotic-resistant strains of haemophilus influenzae, Staphylococcus aureus, and Streptococcus pneumoniae.

 

Mabe S, Champney WS.
Curr Microbiol. 2005 Dec;51(6):363-6. Epub 2005 Oct 25.
PMID: 16252133 [PubMed - indexed for MEDLINE]
Related Articles

9:

Accumulation and turnover of 23S ribosomal RNA in azithromycin-inhibited ribonuclease mutant strains of Escherichia coli.

 

Silvers JA, Champney WS.
Arch Microbiol. 2005 Oct;184(1):66-77. Epub 2005 Nov 3.
PMID: 16096836 [PubMed - indexed for MEDLINE]
Related Articles

10:

Structure-activity relationships for three macrolide antibiotics in Haemophilus influenzae.

 

Mabe S, Eller J, Champney WS.
Curr Microbiol. 2004 Oct;49(4):248-54.
PMID: 15386112 [PubMed - indexed for MEDLINE]
Related Articles

11:

An examination of the differential sensitivity to ketolide antibiotics in ermB strains of Streptococcus pyogenes and Streptococcus pneumoniae.

 

Champney WS, Mentens N, Zurawick K.
Curr Microbiol. 2004 Oct;49(4):239-47.
PMID: 15386111 [PubMed - indexed for MEDLINE]
Related Articles

12:

Neomycin and paromomycin inhibit 30S ribosomal subunit assembly in Staphylococcus aureus.

 

Mehta R, Champney WS.
Curr Microbiol. 2003 Sep;47(3):237-43.
PMID: 14570276 [PubMed - indexed for MEDLINE]
Related Articles

13:

A 50S ribosomal subunit precursor particle is a substrate for the ErmC methyltransferase in Staphylococcus aureus cells.

 

Champney WS, Chittum HS, Tober CL.
Curr Microbiol. 2003 Jun;46(6):453-60.
PMID: 12732954 [PubMed - indexed for MEDLINE]
Related Articles

14:

Bacterial ribosomal subunit assembly is an antibiotic target.

 

Champney WS.
Curr Top Med Chem. 2003;3(9):929-47. Review.
PMID: 12678830 [PubMed - indexed for MEDLINE]
Related Articles

15:

Preferential inhibition of protein synthesis by ketolide antibiotics in Haemophilus influenzae cells.

 

Champney WS, Tober CL.
Curr Microbiol. 2003 Feb;46(2):103-8.
PMID: 12520364 [PubMed - indexed for MEDLINE]
Related Articles

16:

Bacterial ribosomal subunit synthesis: a novel antibiotic target.

 

Champney WS.
Curr Drug Targets Infect Disord. 2001 May;1(1):19-36. Review.
PMID: 12455231 [PubMed - indexed for MEDLINE]
Related Articles

17:

Telithromycin inhibition of protein synthesis and 50S ribosomal subunit formation in Streptococcus pneumoniae cells.

 

Champney WS, Pelt J.
Curr Microbiol. 2002 Nov;45(5):328-33.
PMID: 12232662 [PubMed - indexed for MEDLINE]
Related Articles

18:

The ketolide antibiotic ABT-773 is a specific inhibitor of translation and 50S ribosomal subunit formation in Streptococcus pneumoniae cells.

 

Champney WS, Pelt J.
Curr Microbiol. 2002 Sep;45(3):155-60.
PMID: 12177734 [PubMed - indexed for MEDLINE]
Related Articles

19:

Inhibition of 50S ribosomal subunit assembly in Haemophilus influenzae cells by azithromycin and erythromycin.

 

Champney WS, Miller M.
Curr Microbiol. 2002 Jun;44(6):418-24.
PMID: 12000992 [PubMed - indexed for MEDLINE]
Related Articles

20:

30S ribosomal subunit assembly is a target for inhibition by aminoglycosides in Escherichia coli.

 

Mehta R, Champney WS.
Antimicrob Agents Chemother. 2002 May;46(5):1546-9.
PMID: 11959595 [PubMed - indexed for MEDLINE]
Related Articles Free article in PMC | at journal site