Dr. Philip Musich

Dr. Phil Musich







Phillip Musich, Ph.D.
Professor, Department of Biomedical Sciences

Contact Information:

Room A025 Stanton Gerber Hall (Bldg. 178), VA Medical Center
423-439-2023
Fax"  423-439-2030


Education:

  • 1968 B.S., (cum laude) Chemistry and Biology, Creighton University, Omaha, Nebraska 

  • 1973 Ph.D., Department of Biology, University of Chicago, Illinois

Interdisciplinary Association:

  • Director, Program in Molecular Biology

  • Adjunct Professor of Biological Sciences, College of Arts & Sciences, ETSU

Research Projects:

Eukaryotic genomes are very complex but dynamic structures. We are studying this dynamic flexibility and the role of oxidative DNA damage in aging. As an experimental model system we employ the dynamic L2Hs elements in human DNA and in recombinant DNA constructs. The L2Hs sequences have undergone amplifications and rearrangements in the evolution of human genomes. They are useful in "DNA fingerprinting" individual humans and for following the process of genomic DNA differentiation during the development of cell lineages in embryogenesis and tumor oncogenesis. and in tracking the effects of genomic damage. These properties are related to the potential of L2Hs elements to form unusual DNA structures and to affect the structure of adjacent chromosomal sequences. In a related project, a prokaryotic "replicative evolution" model system has been developed to study the stability of a recombinant plasmid containing these dynamic sequences during normal growth and in response to environmental oxidative challenge.


Selected Publications:

1.

Differential DNA damage responses in p53 proficient and deficient cells: cisplatin-induced nuclear import of XPA is independent of ATR checkpoint in p53-deficient lung cancer cells.

 

Li Z, Musich PR, Zou Y.

 

Int J Biochem Mol Biol. 2011 Apr 15;2(2):138-145.

 

PMID: 21643554 [PubMed] Free PMC Article

 

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2.

Genomic instability and DNA damage responses in progeria arising from defective maturation of prelamin A.

 

Musich PR, Zou Y.

 

Aging (Albany NY). 2009 Jan;1(1):28-37. Review.

 

PMID: 19851476 [PubMed - indexed for MEDLINE] Free PMC Article

 

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3.

Gamma tocopherol upregulates the expression of 15-S-HETE and induces growth arrest through a PPAR gamma-dependent mechanism in PC-3 human prostate cancer cells.

 

Campbell SE, Musich PR, Whaley SG, Stimmel JB, Leesnitzer LM, Dessus-Babus S, Duffourc M, Stone W, Newman RA, Yang P, Krishnan K.

 

Nutr Cancer. 2009;61(5):649-62.

 

PMID: 19838939 [PubMed - indexed for MEDLINE]

 

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4.

Checkpoint kinase ATR promotes nucleotide excision repair of UV-induced DNA damage via physical interaction with xeroderma pigmentosum group A.

 

Shell SM, Li Z, Shkriabai N, Kvaratskhelia M, Brosey C, Serrano MA, Chazin WJ, Musich PR, Zou Y.

 

J Biol Chem. 2009 Sep 4;284(36):24213-22. Epub 2009 Jul 8.

 

PMID: 19586908 [PubMed - indexed for MEDLINE] Free PMC Article

 

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5.

Specific and efficient binding of xeroderma pigmentosum complementation group A to double-strand/single-strand DNA junctions with 3'- and/or 5'-ssDNA branches.

 

Yang Z, Roginskaya M, Colis LC, Basu AK, Shell SM, Liu Y, Musich PR, Harris CM, Harris TM, Zou Y.

 

Biochemistry. 2006 Dec 26;45(51):15921-30. Epub 2006 Dec 19.

 

PMID: 17176115 [PubMed - indexed for MEDLINE] Free PMC Article

 

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6.

Electrotransfer of Long Ranger sequencing gels using a methanol-TBE buffer.

 

Musich PR, Posey EL, Patel A.

 

Biotechniques. 1995 Sep;19(3):382-6. No abstract available.

 

PMID: 7495550 [PubMed - indexed for MEDLINE]

 

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7.

High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay.

 

Li C, Musich PR, Ha T, Ferguson DA Jr, Patel NR, Chi DS, Thomas E.

 

J Clin Pathol. 1995 Jul;48(7):662-6.

 

PMID: 7560176 [PubMed - indexed for MEDLINE] Free PMC Article

 

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8.

A temperature-sensitive mutant of Escherichia coli with an alteration in ribosomal protein L22.

 

Burnette-Vick B, Champney WS, Musich PR.

 

Genetica. 1994;94(1):17-25.

 

PMID: 7729693 [PubMed - indexed for MEDLINE]

 

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9.

A hot alkaline plasmid DNA miniprep method for automated DNA sequencing protocols.

 

Musich PR, Chu W.

 

Biotechniques. 1993 Jun;14(6):958-60.

 

PMID: 8333963 [PubMed - indexed for MEDLINE]

 

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10.

Characterization of an evolutionarily old human alphoid DNA.

 

Carnahan SL, Palamidis-Bourtsos E, Musich PR, Doering JL.

 

Gene. 1993 Jan 30;123(2):219-25.

 

PMID: 8428661 [PubMed - indexed for MEDLINE]

 

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11.

Molecular cloning and characterization of mouse mast cell chymases.

 

Chu W, Johnson DA, Musich PR.

 

Biochim Biophys Acta. 1992 May 22;1121(1-2):83-7.

 

PMID: 1376147 [PubMed - indexed for MEDLINE]

 

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