Dr. Antonio Rusinol

Dr. Antonio Rusinol Antonio Rusinol, PhD
Associate Professor

Stanton-Gerber Hall (VA 178) Room A108
Phone: 423-439-8015

 


Education
Ph.D., 1990, National University of Tucumán, Tucumán, Argentina
B.Sc., 1983, National University of Tucumán, Tucumán, Argentina


Research Interests


My research is focused on two major areas:

A) Elucidation the molecular pathways and mechanisms of the early events of atherogenesis:  The cytotoxic effects of oxLDL have been shown to proceed through apoptotic pathways. Work reported by our group (see bellow ), and others, is consistent with the hypothesis that the induction of apoptosis by oxLDL is mediated by oxysterols. A long term goal of our laboratory is to determine whether macrophage foam cell apoptosis, induced by oxysterols, plays a significant role in atherogenesis.

B) Role of protein prenylation on traffic, function and disease: Protein prenylation can be considered as a mechanism for post-translational attachment of proteins to membranes and targeting signal for intracellular localization . All known prenylated proteins in the cell are found at least under certain conditions, bound to cellular membranes. However, although prenylation clearly mediates membrane association, other factors must determine the specificity of the subcellular localization of particular prenylated proteins since they occur in many diverse subcellular compartments. In some cases this specificity is given by secondary signals in the prenylated proteins (primary structure or additional lipid modifications) whereas in other cases it is the actual interaction with prenylation-specific receptors or protein partners that directs the final localization. The existence of such prenylation-specific binding proteins has been demonstrated by work from our laboratory (1) and work published by others. The function of such proteins is however, not clearly understood. The overall goal of our research is to determine the role of prenylation-specific binding proteins on H-ras and Prelamin A trafficking and function.

 

Selected References

1.

Identification of acidic pH-dependent ligands of pentameric C-reactive protein.

 

Hammond DJ Jr, Singh SK, Thompson JA, Beeler BW, Rusiñol AE, Pangburn MK, Potempa LA, Agrawal A.

 

J Biol Chem. 2010 Nov 12;285(46):36235-44. Epub 2010 Sep 14.

 

PMID: 20843812 [PubMed - indexed for MEDLINE]

 

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2.

Binding of the monomeric form of C-reactive protein to enzymatically-modified low-density lipoprotein: effects of phosphoethanolamine.

 

Singh SK, Suresh MV, Hammond DJ Jr, Rusiñol AE, Potempa LA, Agrawal A.

 

Clin Chim Acta. 2009 Aug;406(1-2):151-5. Epub 2009 Jun 21.

 

PMID: 19545552 [PubMed - indexed for MEDLINE] Free PMC Article

 

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3.

Phosphoethanolamine-complexed C-reactive protein: a pharmacological-like macromolecule that binds to native low-density lipoprotein in human serum.

 

Singh SK, Suresh MV, Prayther DC, Moorman JP, Rusiñol AE, Agrawal A.

 

Clin Chim Acta. 2008 Aug;394(1-2):94-8. Epub 2008 Apr 27.

 

PMID: 18486609 [PubMed - indexed for MEDLINE] Free PMC Article

 

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4.

C-reactive protein-bound enzymatically modified low-density lipoprotein does not transform macrophages into foam cells.

 

Singh SK, Suresh MV, Prayther DC, Moorman JP, Rusiñol AE, Agrawal A.

 

J Immunol. 2008 Mar 15;180(6):4316-22.

 

PMID: 18322245 [PubMed - indexed for MEDLINE] Free PMC Article

 

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5.

Involvement of xeroderma pigmentosum group A (XPA) in progeria arising from defective maturation of prelamin A.

 

Liu Y, Wang Y, Rusinol AE, Sinensky MS, Liu J, Shell SM, Zou Y.

 

FASEB J. 2008 Feb;22(2):603-11. Epub 2007 Sep 11.

 

PMID: 17848622 [PubMed - indexed for MEDLINE] Free PMC Article

 

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6.

Alterations in mitosis and cell cycle progression caused by a mutant lamin A known to accelerate human aging.

 

Dechat T, Shimi T, Adam SA, Rusinol AE, Andres DA, Spielmann HP, Sinensky MS, Goldman RD.

 

Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):4955-60. Epub 2007 Mar 14.

 

PMID: 17360326 [PubMed - indexed for MEDLINE] Free PMC Article

 

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7.

DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.

 

Liu Y, Rusinol A, Sinensky M, Wang Y, Zou Y.

 

J Cell Sci. 2006 Nov 15;119(Pt 22):4644-9. Epub 2006 Oct 24.

 

PMID: 17062639 [PubMed - indexed for MEDLINE] Free PMC Article

 

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8.

Farnesylated lamins, progeroid syndromes and farnesyl transferase inhibitors.

 

Rusiñol AE, Sinensky MS.

 

J Cell Sci. 2006 Aug 15;119(Pt 16):3265-72. Review.

 

PMID: 16899817 [PubMed - indexed for MEDLINE] Free Article

 

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9.

HSPA12B is predominantly expressed in endothelial cells and required for angiogenesis.

 

Steagall RJ, Rusiñol AE, Truong QA, Han Z.

 

Arterioscler Thromb Vasc Biol. 2006 Sep;26(9):2012-8. Epub 2006 Jul 6.

 

PMID: 16825593 [PubMed - indexed for MEDLINE] Free Article

 

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10.

Acyl-coenzyme A:cholesterol acyltransferase promotes oxidized LDL/oxysterol-induced apoptosis in macrophages.

 

Freeman NE, Rusinol AE, Linton M, Hachey DL, Fazio S, Sinensky MS, Thewke D.

 

J Lipid Res. 2005 Sep;46(9):1933-43. Epub 2005 Jul 1.

 

PMID: 15995174 [PubMed - indexed for MEDLINE] Free PMC Article

 

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11.

Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24.

 

Corrigan DP, Kuszczak D, Rusinol AE, Thewke DP, Hrycyna CA, Michaelis S, Sinensky MS.

 

Biochem J. 2005 Apr 1;387(Pt 1):129-38.

 

PMID: 15479156 [PubMed - indexed for MEDLINE] Free PMC Article

 

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12.

AKT/protein kinase B regulation of BCL family members during oxysterol-induced apoptosis.

 

Rusiñol AE, Thewke D, Liu J, Freeman N, Panini SR, Sinensky MS.

 

J Biol Chem. 2004 Jan 9;279(2):1392-9. Epub 2003 Oct 14.

 

PMID: 14559920 [PubMed - indexed for MEDLINE] Free Article

 

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13.

Arachidonate metabolism and the signaling pathway of induction of apoptosis by oxidized LDL/oxysterol.

 

Panini SR, Yang L, Rusinol AE, Sinensky MS, Bonventre JV, Leslie CC.

 

J Lipid Res. 2001 Oct;42(10):1678-86.

 

PMID: 11590225 [PubMed - indexed for MEDLINE] Free Article

 

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14.

Isolation of a somatic cell mutant resistant to the induction of apoptosis by oxidized low density lipoprotein.

 

Rusiñol AE, Yang L, Thewke D, Panini SR, Kramer MF, Sinensky MS.

 

J Biol Chem. 2000 Mar 10;275(10):7296-303.

 

PMID: 10702300 [PubMed - indexed for MEDLINE] Free Article

 

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15.

Translocational pausing of apolipoprotein B can be regulated by membrane lipid composition.

 

Rusiñol AE, Hegde RS, Chuck SL, Lingappa VR, Vance JE.

 

J Lipid Res. 1998 Jun;39(6):1287-94.

 

PMID: 9643361 [PubMed - indexed for MEDLINE] Free Article

 

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16.

Role of lipoproteins in the delivery of lipids to axons during axonal regeneration.

 

de Chaves EI, Rusiñol AE, Vance DE, Campenot RB, Vance JE.

 

J Biol Chem. 1997 Dec 5;272(49):30766-73.

 

PMID: 9388216 [PubMed - indexed for MEDLINE] Free Article

 

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17.

In vitro reconstitution of assembly of apolipoprotein B48-containing lipoproteins.

 

Rusiñol AE, Jamil H, Vance JE.

 

J Biol Chem. 1997 Mar 21;272(12):8019-25.

 

PMID: 9065474 [PubMed - indexed for MEDLINE] Free Article

 

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18.

Monomethylethanolamine reduces plasma triacylglycerols and apolipoprotein B and increases apolipoprotein A-I rats without induction of fatty liver.

 

Rusiñol AE, Lysak PS, Sigurdson GT, Vance JE.

 

J Lipid Res. 1996 Nov;37(11):2296-304.

 

PMID: 8978481 [PubMed - indexed for MEDLINE] Free Article

 

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19.

Apolipoprotein B sequence requirements for hepatic very low density lipoprotein assembly. Evidence that hydrophobic sequences within apolipoprotein B48 mediate lipid recruitment.

 

McLeod RS, Wang Y, Wang S, Rusiñol A, Links P, Yao Z.

 

J Biol Chem. 1996 Aug 2;271(31):18445-55.

 

PMID: 8702489 [PubMed - indexed for MEDLINE] Free Article

 

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20.

Inhibition of secretion of truncated apolipoproteins B by monomethylethanolamine is independent of the length of the apolipoprotein.

 

Rusiñol AE, Vance JE.

 

J Biol Chem. 1995 Jun 2;270(22):13318-25.

 

PMID: 7768932 [PubMed - indexed for MEDLINE] Free Article

 

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21.

A unique mitochondria-associated membrane fraction from rat liver has a high capacity for lipid synthesis and contains pre-Golgi secretory proteins including nascent lipoproteins.

 

Rusiñol AE, Cui Z, Chen MH, Vance JE.

 

J Biol Chem. 1994 Nov 4;269(44):27494-502.

 

PMID: 7961664 [PubMed - indexed for MEDLINE]

 

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22.

Movement of apolipoprotein B into the lumen of microsomes from hepatocytes is disrupted in membranes enriched in phosphatidylmonomethylethanolamine.

 

Rusiñol AE, Chan EY, Vance JE.

 

J Biol Chem. 1993 Nov 25;268(33):25168-75.

 

PMID: 8227080 [PubMed - indexed for MEDLINE]

 

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23.

Impaired biosynthesis of phosphatidylcholine causes a decrease in the number of very low density lipoprotein particles in the Golgi but not in the endoplasmic reticulum of rat liver.

 

Verkade HJ, Fast DG, Rusiñol AE, Scraba DG, Vance DE.

 

J Biol Chem. 1993 Nov 25;268(33):24990-6.

 

PMID: 8227061 [PubMed - indexed for MEDLINE]

 

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24.

Assembly of rat hepatic very low density lipoproteins in the endoplasmic reticulum.

 

Rusiñol A, Verkade H, Vance JE.

 

J Biol Chem. 1993 Feb 15;268(5):3555-62.

 

PMID: 8429031 [PubMed - indexed for MEDLINE]

 

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25.

Estrogen treatment increases phospholipid transfer activities in chicken liver.

 

Rusiñol AE, Bloj B.

 

J Biol Chem. 1989 Apr 25;264(12):6612-4.

 

PMID: 2708326 [PubMed - indexed for MEDLINE]

 

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26.

Phospholipid transfer activities in toad oocytes and developing embryos.

 

Rusiñol A, Salomón RA, Bloj B.

 

J Lipid Res. 1987 Jan;28(1):100-7.

 

PMID: 3104520 [PubMed - indexed for MEDLINE] Free Article

 

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