Resources

Shared Reagents:

Kumar, Dhirendra Ph.D.- 423-439-5958

  • Cell suspension cultures from Tobacco (Nicotiana tabacum)
  • anit-myc monoclonal antibodies

The following sites are excellent for anyone who is a novice at pipetting, or someone who has never been properly trained.

Micropipetting techniques:

http://www.cabrini.edu/sepchedna/2002%20Workshop/Labs/measurements_and_micropipetting.htm

http://www.sigmaaldrich.com/life-science/cell-culture/learning-center/cell-culture-videos/the-micropipette.html

The following video provides basic information on preparation of an agarose gel:

Agarose gel preparation:

mms://etsumedia2.etsu.edu/agarose_gel


Basic information on ligation/ transformation and plating can be found in the following video.

Cloning and plating:

 

mms://etsumedia2.etsu.edu/cloning_and_plating

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