See www.chem.agilent.com "Lab-on-a-Chip" for sample specifications and details
The Agilent Bioanalyzer is a microfluidics-based platform for rapid analysis of DNA, RNA, and Protein.
How Does it Work?
How To Use the Bioanalyzer
A. Molecular Biology Core Facility Can Run Your Samples For You
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Submit your samples, fill out user form CLICK HERE for Agilent Order Form
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Contact Core before 1st run to discuss sample submission
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Core will run the samples, send you the data
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Fee: cost of chip plus labor
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When possible the Core will run multiple investigator samples on same chip and divide cost accordingly
B. You May Run Your Own Samples
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Lab must buy a box of chips/reagents from the MBCF
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NO SHARING AMONG LABS
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No additional fees
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MBCF will train a dedicated user in that lab when the kit is purchased
Steps in a Bioanalyzer Analysis:
1. Prepare gel-dye mixture
–Enough for several chips
2. Prepare samples
–DNA samples – no prep
–RNA samples – heat at 70°C
–Protein samples – heat at 95°C
3. Load chip with gel-dye mixture
–Prime chip
4. Load samples/ladder on chip
–Sample
–Loading buffer
5. Internal control marker
6. Place chip in Bioanalyzer and hit “Start”
7. Come back in 30 minutes for your data
RNA Integrity Number (RIN):
Fee Schedule:
Agilent Bioanalyzer Kits are discounted 5% off list price when ordered through the MBCF!
The following kits are kept in the Core Facility for user submission.
Contact us for details.
RNA NANO: MBCF run: $38.00/chip
DNA1000: MBCF run: $32.00/chip