HSCI 4771                Virology Laboratory        Spring 2002

No text. Reading materials will be placed on reserve at Sherrod Library. Students should take notes on the materials as they read them and prepare for class discussions.

Course web page URL: http://www.etsu.edu/cpah/hsci/hsci4770.html

Videos. We will view several videos during the semester. You should take extensive notes on these videos as you view them. Record facts, details, and questions. Because most of these videos run 50-55 minutes, we will have to hold our discussions until the specified days. Consequently, your notes are critical. Review your notes to prepare for the class discussion sessions. If you miss a video there is NO opportunity to make it up. Mark that youwere absent in your notebook even if you copy notes from a classmate.

Wet Labs. During the semester we will grow bacterial viruses (phage) and plant viruses (TMV). Also, we will culture animal cells that are commonly used to grow animal viruses. Unfortunately, we do not have the appropriate facilities to safely grow animal viruses.

Lab Notebook/Journal: Students should keep a log of notes on the videos, readings, discussions and experiments conducted during the laboratory time. All observations and materials related to the lab should be kept in thisnotebook. Put anything about virology you want to include in this notebook.You can include news articles, web pages, popular magazine articles, andpictures, etc. Absences should be recorded.
Notebooks will be graded twice.
Criteria for grading notebooks

• Organization and Appearance – Table of contents included? Does it look interesting? Can you find things?
• Thoroughness / completeness – Did you take a lot of notes on the videos, readings, discussions?
• Accuracy – Is everything correct/true?
• Mechanics – Is thespelling, grammar, sentence structure correct? (Do the subjects and verbsagree? This is very important in the real world. It is correct to say scientistsstudy or a scientist studies. It is incorrect to say scientists studies anda scientist study.)

Grades:
·    Lab Notebook/Journal ( 2 X 25%)………………………..…………….…………50%                                                                 
·    Exams (2 X 25% )…………………………………………………………………… 50%
·   
All Makeup exams will be essay questions administered on Friday, April 27 at 9:20.


Final semester grades will be assigned as follows:
93-100% = A    88-89%  = B+    78-79% = C+        67-69% = D+        <60%   = F
90-92%   = A-    83-87%  = B      73-77% = C        60-66% = D
       80-82%  = B-    70-72% = C-   
              
Attendance policy: Students are expected to come to class on timeand prepared. Attendance will be recorded at every class session. You have3 free absences; no explanation required. Your 4th absence will lower yourgrade 5%. Each subsequent absence will lower your grade an additional 5%.Each tardy counts as 1/3 an absence.

Date                 Laboratory Activities


M Jan 8    Introduction / Icosahedrals

W Jan 10    Molecular Biology Software – Before Feb 19 visit the Health Sciences Computer Lab, room 236, to use the softwareon your own. It will open Jan 22. Use software for at least 30 minutes during a visit.

M Jan 15        MLK Holiday

W Jan 17    Video -Virus Hunters 
Required Reading on Reserve at Sherrod Library:
1.    C.J. Peters Ch 19 Experiences of a Virus Hunter. Many Faces, Many Microbes. Editor Ronald M. Atlas. ASM Press 2000 p146-157
2.    Sir Michael Anthony Epstein: “An Obsessional Sort of Temperment” and the Epstein-Barr Virus. Disease Fighters Since 1950. Editors Ray Spangenburg and Diane K. Moser. Facts On File, Inc. 1996, p48-57.
3.    Nobel Prize in Chemistry 1946 – Wendell Stanley (presentation speech)Nobel Prize Web Page http://www.nobel.se

M Jan 22    Ch 36 Laboratory Diagnosis  
Required Reading on Reserve at Sherrod Library:
4.    Zimmerman, Barry and David. Ch 8 Common Viruses that Kill. Killer Germs. Contemporary Books, Inc.1996, p153-171.

W Jan 24    DiscussionPrepare yourself by reading the assignments and reviewing your notes on the videos. Ask any questions you have regarding the laboratory material, or your notebook. If time permits, we may discuss material covered in lecture exam 1.

M Jan 29    Video- Outbreak 1918

W Jan 31    Video- 1918 Flu Pandemic

M Feb 5    Video- Hunt for the Killer Flu

W Feb 7    Video- The Body Story: Influenza B

M Feb 12    Video- Smallpox: Deadly Again? 
Required Reading on Reserve at Sherrod Library:
5.    Bernard Dixon. Smallpox virus. Power Unseen. W.H. Freeman & Co.1996, p35-38
6.    Wayne Biddle. Smallpox. A Field Guide to Germs. Henry Holt & Co.1996, p127-134

W Feb 14    Video- Polio Vaccine  Required Readingon Reserve at Sherrod Library:
7.    Jonas Salk and Albert Bruce Sabin: Preventing Polio. Disease Fighters Since 1950. Editors Ray Spangenburg and Diane K. Moser. Facts On File, Inc. 1996, p58-75.
8.    Wayne Biddle. Polio. A Field Guide to Germs. Henry Holt & Co. 1996, p111-115.

M Feb 19    Discussion Prepare yourself by reading the assignments and reviewing your notes on the videos. Ask any questions you have regarding the laboratory material, your notebook or the exam.

W Feb 21    Mid-Term Exam / Notebook due

M Feb 26    Video-The Virus that Cures

W Feb 28    Phage lab

Required Reading on Reserve at Sherrod Library:  
9. Nobel Prize in Physiology or Medicine 1969 Max Delbruck, Alfred Hershey and Salvador E. Luria (press release)
M Mar 5    Phage lab
W Mar 7    Phage lab

M Mar 12    Spring Break
W Mar 14    Spring Break

M Mar 19    Phage  and Tobacco Mosiac Virus lab - Handout

W Mar 21    Overview of PCR  Required Reading on Reserve at Sherrod Library:
10.    Kary B. Mullis. The Unusual Origin of the Polymerase Chain Reaction. Scientific American. April 1990. P 56-65.

M Mar 26    Tissue Culture – Handout 
Required Reading on Reserve at Sherrod Library:
11. Nobel Prize in Physiology or Medicine 1951 – Max Theiler (presentation speech)
12. Nobel Prize in Physiology or Medicine 1954 – John Franklin Enders, Thomas Huckle Weller and Fredrick Chapman Robbins (presentation speech)

W Mar 28    TMV
Required Reading on Reserve at Sherrod Library:
13.    Sulzinski, Michael A. The Tobacco Mosaic Virus. The Science Teacher. May 1992, p42-45.1
4.    Maratos, Marina and Larry McDaniel. Tobacco Mosaic Virus: A Research and Education Tool for the 21st Century. ATCC Quarterly Newsletter. Vol 14. No. 3 & 4. 1994, p1, 9-10.

M April 2    Video – Ebola: The Plague Killers

W April 4    Video – Killer Viruses

M April 9    Video – HBV Vaccine On Trial
15. Nobel Prize in Physiology or Medicine 1976 – Baruch S. Blumberg (HBV) press release

W April 11    Video-HIV 
Required Reading on Reserve at Sherrod Library:
16.    Francoise Barre-Sinoussi: The Virus that Causes AIDS. Disease Fighters Since 1950. Editors Ray Spangenburg and Diane K. Moser. Facts On File, Inc. 1996, 115-129.

M April 16    Video -HIV

W April 18    Video-Mad Cow Disease 
Required Reading on Reserve at Sherrod Library:
17.    Nobel Prize in Physiology or Medicine 1976 – Carleton Gajdusek (kuru) press release
18.    Nobel Prize in Physiology or Medicine 1997 – Stanley B. Pruisner press release

M April 23    Discussion

W April 25    Final Exam / Notebook due