Lok R. Pokhrel

Pokhrel LokRajLok R. Pokhrel
Ph.D. Candidate
Environmental Health
Lamb Hall, Room 65B
Phone: 423-439-5258
Email:
Personal Blog: http://lokpokhrel.blogspot.com/

Education:

Ph.D. East Tennessee State University, Johnson City, TN, USA, May 2013 (due) Nanotoxicology /ENVH (Co-Advisors: Drs. Phillip Scheuerman, Brajesh Dubey)

M.S. East Tennessee State University, Johnson City, TN, USA, 2009 Quantitative Biology (Advisor: Dr. Istvan Karsai)

M.Sc. Degree Campus, Tribhuvan University, Nepal, 2001 Zoology

B.Sc. Mechi Multiple College, Tribhuvan University, Nepal, 1999 Zoology

Research interests:

Understanding the potential fate and toxicology of engineered nanomaterials in the environment

Nanotechnology is touted to bring another industrial revolution as hundreds of products containing nanomaterials are available in various sectors including medical diagnostics and therapeutics to personal care products, light and strong materials for automotives to non-odorous clothing, and anti-fouling membranes for water purification. With advances in nanotechnology, environmental health and safety (EHS) issues of man-made nanoparticles are also growing as nanoproducts release nano-sized particles (1nm = 1 billionth of a meter) into the environment. My research aims at understanding the fate and transformation of metal nanoparticles in the environmental media and how changes in particle behavior could influence the toxicity in various biologic milieus. My work has involved working with lab-synthesized and well-characterized metal-based engineered nanoparticles such as silver nanoparticles, zinc oxide nanoparticles, titanium dioxide nanoparticles, and quantum dots. Various model organisms such as Escherichia coli, Daphnia magna, Odonate nymphs, Zea mays, and Brassica oleraceae var. capitata have been utilized to investigate the research problem.

Biography:

I grew up in a small town of Bhadrapur in Southeast of Nepal—the country known for its natural beauty and the Himalayas. I did my Kindergarten through Undergraduate studies in my home town before I moved to a big city of Biratnagar for my graduate studies. I then moved to the capital city, Kathmandu, to teach environmental health and biology at Budhanilkantha School, the nation’s center of excellence. In 2007, I arrived at ETSU in pursuit of higher studies. Currently, I am a Ph.D. candidate in the Department of Environmental Health. Choosing Environmental Health field has been rewarding for me as my research seeks to understand the potential mechanistic effects of emerging contaminants including the anthropogenic nanomaterials to the environment, with an ultimate goal of developing a knowledge base that could be helpful in making safer living environment.

Something Intresting:

I can make ginger out of my fingers! I love playing soccer and cricket, and enjoy watching boxing and UFC in spare times.

Publications:

1. Pokhrel LR, Dubey B. Potential impact of low-concentration silver nanoparticles on predator-prey interactions between predatory dragonfly nymphs and Daphnia magna as a prey. ACS Environmental Science & Technology 2012, 46(14):7755-7762.

2. Pokhrel LR, Dubey B, Silva T, El Badawy AM, Tolaymat TM, Scheuerman PR. Rapid screening of aquatic toxicity of several metal-based nanoparticles using the MetPLATE™ bioassay. Science of the Total Environment 2012, 426:414-422.

3. Pokhrel LR, Dubey B. (2012) Global scenarios of metal mining, environmental repercussions, public policies, and sustainability: A review. Critical Reviews in Environmental Science and Technology DOI:10.1080/10643389.2012.672086.

4. Pokhrel LR, Karsai I, Laughlin TF, Hamed MK. Dorsal body pigmentation and sexual dimorphism in the marbled salamander Ambystoma opacum. Ethology, Ecology and Evolution ( revision submitted, Manuscript # TEEE-2012-0017-R).

5. Pokhrel LR, Dubey B. Engineered nanomaterials in the waste disposal and treatment systems: Potential fate, effects and the regulatory conundrum. Journal of Environmental Monitoring (Invited Review, under review ).

6. Pokhrel LR, Dubey B. Silver and zinc oxide nanoparticles cause developmental anomalies in the crop plants. Environment International (to be submitted soon).

7. Pokhrel LR, Silva TU, Dubey B, Tolaymat TM, Maier KJ, Scheuerman PR. General Linear Model-predicted and observed toxicity of organic-coated silver nanoparticles to both prokaryotic and eukaryotic organisms: Probing interactive effects of particle size and surface charge. Nano Letters (to be submitted soon).

8. Pokhrel LR, Karsai I. Long-term effects of low-concentration environmental drift of Roundup herbicide on the development and fecundity of an invasive weed, Bryophyllum pinnatum. For Science of the Total Environment (to be submitted soon).

 

BOOKS

Pokhrel LR , Acharya HN (2008) Population and Environment Education - Grade VI, VII, & VIII. Vidyarthi Publications , Kathmandu, Nepal (Text Books for Junior School in Nepal).

 

CONFERENCE PRESENTATIONS (abstract published)

1. Dubey B, Pokhrel LR. (2012) Opportunities for nanoparticle research. Invited presentation at University Consortium for Field-Focused Groundwater Contamination Research Program, Annual Progress Meeting: June 12-14, 2012, The Arboretum, University of Guelph, ON, Canada.

2. Pokhrel LR, Dubey B, Scheuerman PR. (2012) Developmental phytotoxicity of silver and zinc oxide nanoparticles to the crop plants. NEHA Conference, June 28-30, San Diego, CA. (Winner of NEHA/CDC/AEHAP Student Competition Research Award 2012).

3. Pokhrel LR, Scheuerman PR, Dubey B. (2012) Ecological risk assessment of zinc oxide nanoparticles. Proceedings of the 2nd International Conference on Environmental Pollution and Remediation, Montreal, Quebec, Canada, August 28-30 (full length paper).

4. Pokhrel LR, Dubey B. (2012)Metal mining industry: Global production statistics, potential environmental effects, mitigation techniques, and sustainability. 13th Intl Symposium on Environmental Issues and Waste Management in Energy and Mineral Production SWEMP 2012, November 28-30, New Delhi, India (full length paper).

5. Pokhrel LR, Dubey B. (2012) Potential developmental toxicity of silver and zinc oxide nanoparticles to the terrestrial plants. 28th Int'l Conference on Soil, Sediment, Water, and Energy, October 15-18, Amherst, MA.

6. Pokhrel LR, Scheuerman PR, Tolaymat TM, Dubey B. (2012) Potential impacts of metal nanoparticles on the growth and development of crop plants. Appalachian Student Research Forum, April 5,Johnson City, TN.

7. Pokhrel LR, Dubey B. (2012) Silver and zinc oxide nanoparticles cause developmental anomalies in the terrestrial plants. Tennessee Environmental Conference, March 13-14, Kingsport, TN.

8. Pokhrel LR, Dubey B, Singhal N. (2012) Assessing the environmental risks from road marking glass beads. Tennessee Environmental Conference, March 13-14, Kingsport, TN.

9. Pokhrel LR, Silva T, El Badawy AM, Tolaymat TM, Dubey B. (2011) Evaluation of aquatic toxicity of nanoscale silver, zinc oxide, titanium dioxide and cadmium selenide quantum dots, and their ionic particulates to the MetPLATE™ bioassay. 27th Int'l Conference on Soil, Sediment, Water, and Energy, October 17-20, Amherst, MA (Winner of Adventus Americas Best Platform Presentation Award).

10. Pokhrel LR, Silva TU, El Badawy AM, Tolaymat TM, Dubey B. (2011) Evaluation of aquatic toxicity of nanoscale silver, zinc oxide, titanium dioxide and cadmium selenide quantum dots, and their ionic particulates to the MetPLATE™ bioassay. Gordon Research Conference, May 29-June 3, New Hampshire, NH.

11. Pokhrel LR, Silva TU, El Badawy AM, Tolaymat TM, Dubey B. (2011) Evaluation of aquatic toxicity of nanoscale silver, zinc oxide, titanium dioxide and cadmium selenide quantum dots, and their ionic particulates to the MetPLATE™ bioassay. Appalachian Student Research Forum, March 24, Johnson City, TN.

12. Pokhrel LR, Dubey B, Scheuerman PR. (2011) Pharmaceuticals in the aquatic environment: A critical appraisal of uncertainty and knowledge gaps in human health risk assessment. NESAJ PATRA 8(1):4-13.

13. Pokhrel LR, Dubey B. (2011) Nanoecotoxicity and human health: Where do we stand? Appalachian Student Research Forum, March 24, Johnson City, TN.

14. Pokhrel LR, Dubey B. (2011) Nanoecotoxicity and human health: Where do we stand? Tennessee Environmental Conference, March 15-16, Kingsport, TN.

15. Pokhrel LR, Dubey B. (2010) Investigation of the environmental fate, transport, and transformation of silver nanoparticles in the waste disposal systems. Appalachian Student Research Forum, April 8, Johnson City, TN.

16. Pokhrel LR, Karsai I. (2008) Mapping the skin pattern of spotted salamanders, Ambystoma maculatum. Appalachian Student Research Forum, April 2-3, Johnson City, TN (Winner of the First Place Award).