Student Reseach

Abstracts from the 2013 Appalachian Student Research Forum can be viewed here.

Titles and authors of the poster presentations:

 

EXPRESSION OF RECOMBINANT HUMAN NEUTROPHIL CATHEPSIN G 
 
Evan Perry1, Eliot Smith1, and Dr. David Johnson1,2,
1Department of Biomedical Sciences, Quillen College of Medicine and 2 Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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OPTIMUM CONDITIONS FOR ENTEROPEPTIDASE EXPRESSION IN PICHIA PASTORIS

Megan Brittany Sears1, Eliot T. Smith1, and David A. Johnson1,2.
1 Department of Biomedical Sciences, Quillen College of Medicine and Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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SENESCENCE IN HUTCHINSON-GILFORD PROGERIA SYNDROME CELLS RELATIVE TO DNA REPLICATION ACTIVITY AND DNA DAMAGE ACCUMULATION
 
Henry Gong1 and Dr. Phillip Musich2,3
1 Department of Biological Sciences, College of Arts and Sciences,
2 Department of Biochemistry and Molecular Biology, Quillen College of Medicine,
Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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RECOMBINATION AND SCREENING OF PUTATIVE GRAPEFRUIT GLUCOSYLTRANSFERASE 4 IN PICHIA PASTORIS

Peri Loftis1 and Cecilia A. McIntosh1,2
1 Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
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FIGHTING FEMALE FLESH FLIES: A STUDY OF BEHAVIORAL ONTOGENY IN THE FLESH FLY SARCOPHAGA CRASSIPALPIS

Anthony Lundy1 , Veronica Fregoso1, Darrell Moore1,3, Mark Phillips1, Edith Seier2,3and Karl H. Joplin1,3
1Department of Biological Sciences
2Department of Mathematics and Statistics
3Institute for Quantitative Biology
College of Arts and Sciences, East Tennessee State University, Johnson City, TN
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MULTIDIMENSIONAL ANALYSIS OF SOLITON SOLUTIONS TO A PERTURBED NONLINEAR SCHRODINGER EQUATION 

Erin Middlemas1,3 and Jeff Knisley1,2
1Department of Mathematics and Statistics
2Institute for Quantitative Biology
3Department of Physics
College of Arts and Sciences, East Tennessee State University, Johnson City, TN
 
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TEMPORAL CHANGES IN SPATIAL POSITIONING FOR FORAGING OPTIMIZATION IN THE SUBSOICAL SPIDER ANELOSIMUS STUDIOSUS (ARANEAE: THERIDIIDAE)
 
Chelsea R. Ross1 ,2 , J. Colton Watts1 , Dr. Edith Seier 2, 3 , Dr. Michele Joyner 2,3 , Dr. Thomas C. Jones 1,3
1Department of Biological Sciences and 2Department of Mathematics and Statistics
College of Arts and Sciences,
3Institute for Quantitative Biology
 East Tennessee State University, Johnson City, TN
 
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GRAPH ALGORITHMS FOR LARGE NETWORKS USING MAPREDUCE
 
Eric Seguin1 , Steve Keller2, and Dr. Jeff Knisley1,3
1 Department of Mathematics and Statistics, College of Arts and Sciences
2 Department of Computer Science, College of Business and Technology
3Institute for Quantitative Biology,
East Tennessee State University, Johnson City, TN
 
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PUTATIVE GLUCOSYLTRANSFERASE 11 FROM CITRUS PARADISI: CLONING, RECOMBINANT EXPRESSION IN YEAST, AND SUBSTRATE SCREENING

Bruce E. Williams1 and Dr. Cecilia A. McIntosh1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University Johnson City, TN
 
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ELECTED POINT MUTATION OF A FLAVONOID 3-O-GLUCOSYLTRANSFERASE FROM CITRUS PARADISI (GRAPEFRUIT) AND ITS EFFECT ON SUBSTRATE AND REGIOSPECIFICITYR

Olusegun A. Adepoju1, Dr. Shiva Devaiah1 and Dr. Cecilia McIntosh1,2
1Department of Biological Sciences
Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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ROLE OF RD22 LIKE PROTEIN (SBIP-355) IN SALICYLIC ACID MEDIATED PATHWAY IN PLANTS PROTEIN
 
Hanadi Almazroue1 and Dr. Dhirendra Kumar1,2
1Department of Biological Sciences,
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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CHARACTERIZATION OF A PUTATIVE PHOSPHOLIPASE  D- δ  PROTEIN IN SALICYLIC ACID MEDIATED DEFENSE
PATHWAY IN TOBACCO   
 
Phillip Dean1 and Dr. Dhirendra Kumar1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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INTERACTION BETWEEN SABP2 AND SBIP24 AND ITS EFFECT ON SA MEDIATED SIGNALING PATHWAY IN PLANTS


Amin Jannatul Ferdous1 and Dr. Dhirendra Kumar1,2
Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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ANT COLONY ENERGY DYNAMICS: AN AGENT-BASED MODEL OF THE ENERGY FLOW

Xiaohui Guo1 and Istvan Karsai1,2
Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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SBIP-350 AND ITS EFFECT ON SABP2 AND SALICYLIC ACID-MEDIATED PATHWAY

Chrisshawnda Johnson1 and Dr. Dhirendra Kumar1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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SITE-DIRECTED MUTAGENESIS IN CITRUS PARADISI-SPECIFIC 3-O-GLUCOSYLTRANSFERASE

Sara S. Khaja1, Dr. Shivakumar P. Devaiah1, and Dr. Cecilia A. McIntosh1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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PHENOTYPIC PLASTICITY AND FLUCTUATING ASYMMETRY VARIATIONS IN LEAF MORPHOLOGY OF THREE

QUERCUS SPECIES IN RESPONSE TO ENVIRONMENTAL FACTORS

Joseph Kusi1 and Dr. Istvan Karsai1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
 
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COLOGICAL IMPLICATIONS OF DIEL RHYTHM OF AGGRESSION IN THE SUBSOCIAL SPIDER ANELOSIMUS STUDIOSUS (ARANEAE: THERIDIIDAE)
 
J. Colton Watts1 , Chelsea R. Ross1,2, Edith Seier2,3, Michele L. Joyner2,3, and Thomas C. Jones1,3.
1 Department of Biological Sciences
2Department of Mathematics and Statistics
College of Arts and Sciences
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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PREVALENCE OF VITAMIN D DEFICIENCY AND INSUFFICIENCY

Yan Cao1 and Xuefeng Liu1,2
1Department of Biostatistics and Epidemiology, College of Public Health
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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DIABETES MELLITUS AND UNCONTROLLED BLOOD PRESSURE: THE ASPECT OF RACIAL DISPARITY IN DIAGNOSED HYPERTENSIVE PATIENTS
Joshua Longcoy1, Joseph C Ikekwere1, and Xuefeng Liu1,2
1Department of Biostatistics and Epidemiology, College of Public Health
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
 
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SITE-DIRECTED MUTATIONAL ANALYSIS
OF FLAVONOL-3-O-GLUCOSYLTRANSFERASES FROM CITRUS PARADISI
 
Shivakumar P. Devaiah1 and Dr. Cecilia A. McIntosh1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN 
 
 
 
STABILITY AND RESILIENCE OF CHAOTIC SYSTEMS OF COUPLED OSCILLATORS
 
Allison Leich Hilbun1and Dr. Istvan Karsai2,3 ,
1 Department of Biomedical Sciences, Quillen College of Medicine
2 Department of Biological Sciences, College of Arts and Sciences
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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TRENDS OF OCCUPATIONAL LYME DISEASE IN MAINE (1999-2011)

Kate Callahan1, Megan Saunders2, Colleen Scott4, Dr. Shimin Zheng1,3
1Department of Biostatistics and Epidemiology, College of Public Health
2Department of Community and Behavioral Health, College of Public Health
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
4Department of Infectious Disease Epidemiology
Maine Center for Disease Control and Prevention, Augusta, ME
 
 
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RISK FACTORS FOR HEART DISEASE IN PATIENTS WITH TYPE 2 DIABETES: RACE DIFFERENCES

Yi He1, Liang Wang1, and Dr. Ke-Sheng Wang1,2
1Department of Biostatistics and Epidemiology, College of Public Health
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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RACIAL DIFFERENCES IN RISK FACTORS FOR THE PREVALENCE OF OBESITY IN US ADULTS
 
Marc Stevens, Jr.1, Dr. Liang Wang1, Dr. Kesheng Wang1,3 , and Dr. Jodi Southerland2
1Department of Biostatistics and Epidemiology, College of Public Health
2Department of Community and Behavioral Health, College of Public Health
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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CESAREAN SECTION AND THE RISK OF OVERWEIGHT IN GRADE SIX CHILDREN
 
Marc Stevens, Jr.1 , Dr. Liang Wang1 , Dr. Arsham Alamian1, Dr. Jodi Southerland2,Dr. Kesheng Wang1,3, and Dr. James Anderson1,3
1Department of Biostatistics and Epidemiology,
2Department of Community and Behavioral Health
College of Public Health
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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DIFFERENTIAL CELLULAR UPTAKE OF TWO FLAVONE ISOMERS

Timothy LeJeune1 , Kayla Pearson1, Hei Yin Tsui1, Julie Wheeler1, Maria Ballester2 Ruben D. Torrenegra3, Oscar E. Rodriguez3 , and Victoria P. Ramsauer1,4

1Department of Pharmaceutical Sciences, Gatton College of Pharmacy
4Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
2Division of Math, Science and Technology, Farquhar College of Arts and Sciences, Nova Southeastern University, Fort Lauderdale, FL
3Departamentos de Quimica y Quimica Farmaceutica,
Universidad de Ciencias Aplicadas y Ambientales, Bogota, Colombia
 
 
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FLAVONE B MECHANISM OF ACTION

Kayla Pearson, Timothy LeJeune, Brian McPherson, Irene Tsui, Christopher Livesay, Gerry Miller,
Brooke Adams, Sierra Street, Julie Wheeler, Oscar Rodriguez, Ruben D. Torrenegra and
Victoria P. Ramsauer, Department of Pharmaceutical Sciences, Gatton College of Pharmacy,
East Tennessee State University, Johnson City, TN
 
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TOPICAL ACHYROCLINE EXTRACT DEMONSTRATES METHICILLIN-RESISTANCE
STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS PYOGENES BACTERICIDAL ACTIVITY
Rachel Sharpton¹, Brian McPherson¹, Morgan H. Pendleton¹, Ruben D. Torrenegra², Oscar E. Rodriguez²,
Donald A. Ferguson Jr.³, and Victoria P. Ramsauer¹,4
¹Department of Pharmaceutical Sciences, Gatton College of Pharmacy
East Tennessee State University, Johnson City, TN
²Universidad de Ciencias Aplicadas y Ambientales, Bogota, Colombia
³Department of Microbiology, Quillen College of Medicine,
4Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
 
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SELECTIVE CYTOTOXICITY OF ASTERACEAE FAMILY EXTRACTS
ON PANCREATIC, COLON, AND LUNG CANCER
 
Hei Yin Tsui1 , Kayla Pearson1 , Timothy LeJeune1 , Brian McPherson1 , Gerry Miller1 , Sierra Street1 ,
Julie Wheeler1, Christopher Livesay1, Brooke Adams1, Ruben Torrenegra3, and Victoria Ramsauer1,2
1Department of Pharmaceutical Sciences, Gatton College of Pharmacy
2Institute for Quantitative Biology
East Tennessee State University,
Johnson City, TN
3Universidad de Ciencias Aplicadas y Ambientales
Bogota, Colombia
 
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RACE DIFFERENCES IN THE ASSOCIATIONS OF SMOKING AND ALCOHOL CONSUMPTION WITH THE RISK OF TYPE 2 DIABETES

Min Zeng1 and Kesheng Wang1,2
Department of Biostatistics and Epidemiology, College of Public Health
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN
 
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****Oral Presentations****

ENHANCING GRAPH ALGORITHM USING MAP-REDUCE FOR LARGE NETWORKS
AND POSSIBLE REAL-WORLD APPLICATION

Steven E. Keller1, Dr. Jeff Knisley2,3 , and Eric Seguin2
1 Department Computer Science, College of Business and Technology
2 Department of Mathematics and Statistics, College of Arts and Sciences
3Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN

Graph Algorithms provide valuable information, but on large networks they can be cost prohibitive due to the very large number of nodes in such networks. For example, Dijsktra's algorithm for shortest distance between two nodes of a weighted graph is an efficient depth first search that works well for relatively small networks. For larger networks, however, a breadth-first search using Map-Reduce is preferable because the Map-Reduce algorithm utilizes the resources of a High Performance Computing (HPC) cluster very well. In this project, we examine certain vertex weighted algorithms that have been effective in some bioinformatics and proteomics applications but that are too slow for large networks. The algorithms in this project are chosen because they are also accessible to a Map-Reduce approach, and consequently, will be able to provide the same types of valuable information about larger networks that they have provided for smaller ones already. The implementation is via the networkx package in Python and the use of mpi4py. Our goal is to incorporate entropic measures and their influence in preferential attachment in the analysis of large bimolecular structures. These measures can then be ultimately applied to any real-world network where statistical entropy plays a significant role. One example of a possible real-word application would be determining the shortest path in air traffic patterns, to create optimum paths airlines have to follow for each distance travel.

ROLE OF A PUTATIVE LIPID TRANSFER PROTEIN/SBIP-470 IN SA-MEDIATED DEFENSE PATHWAY

Danda Pani Chapagai1 and Dr. Dhirendra Kumar1,2
1Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN

As wide array of functions of Salicylic Acid (SA) in plant, it involves in growth and development, ion
uptake and transport, photosynthesis, transpiration and defense against pathogens. It plays key role in
pathogen resistance through gene expression of Pathogen Related (PR) proteins. Subsequently, plant
acquires Systemic Acquired Resistance (SAR) in which pathogen attacks one part of plant induces
resistance in the other uninfected part. During this process, plant produces high levels of SA which is
converted to Methyl Salicylate (MeSA), a SAR signal. Salicylic Acid Binding Protein 2 (SABP2) catalyzes
conversion of MeSA into SA to induce resistance. Yeast two-hybrid screening using SABP2 as a bait it, a
tobacco protein (SBIP-470) was found to interact with SABP2. Based on various analysis e.g. BLAST,
phylogenic tree and Protein Predict, SBIP-470 belongs to non specific Lipid Transfer Protein 1 (nsLTP1)
subfamily whose members are responsible for transferring of Lipid and its derivatives from inside the cell to developing cuticle with or without the conjunction with SA. In recent years new insights into how
different molecules including Lipid Transfer Protein (LTP) coordinate perception and transmission of SAR
signaling in plant during pathogen resistance has come to light. SA may activate the gene expression of SBIP-470 and the gene product may transfer the Lipid and its derivatives from inside the cell to outside the cell for the cuticle synthesis as the physical barrier for pathogens. In the present study, therefore, we will first characterize the SBIP-470 then investigate the roles of it in SA mediated defense pathway. In order to characterize the SBIP-470, the expression of SBIP-470 in Escherichia coli, affinity purification, in vitro lipid transfer assay and effect of SABP2 and SA/MeSA on lipid transfer activity will be carried out. Likewise, the Arabidopsis knock out mutants which lack SBIP-470 homolog gene will be treated with various plant pathogens. Moreover, the composition and quantity of cuticular wax and cutin of leaves, as well as the stem of both control and mutant plants will be analyzed using the Gas Chromatography -Mass Spectrometry (GC- MS) and Gas Chromatography. Understanding the role of the SBIP-470 in SA mediated pathway can lead to develop the pathogen resistant plant and abridging the use of expensive pesticide and fungicide for healthy world.first characterize the SBIP-470 then investigate the roles of it in SA mediated defense pathway. In order to characterize the SBIP-470, the expression of SBIP-470 in Escherichia coli, affinity purification, in vitro lipid transfer assay and effect of SABP2 and SA/MeSA on lipid transfer activity will be carried out. Likewise, the Arabidopsis knock out mutants which lack SBIP-470 homolog gene will be treated with various plant pathogens. Moreover, the composition and quantity of cuticular wax and cutin of leaves, as well as the stem of both control and mutant plants will be analyzed using the Gas Chromatography -Mass Spectrometry (GC- MS) and Gas Chromatography. Understanding the role of the SBIP-470 in SA mediated pathway can lead to develop the pathogen resistant plant and abridging the use of expensive pesticide and fungicide for healthy world.

LYSINE ACETYLATION, A POSTTRANSCRIPTIONAL MODIFICATION
OF PROTEINS IS INVOLVED IN SALICYLIC ACID MEDIATED SIGNALING PATHWAY

Imdadul Haq1 and Dhirendra Kumar1,2
Department of Biological Sciences, College of Arts and Sciences
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN

Lysine acetylation is an essential and reversible posttranscriptional modification involved in signaling
pathways of both prokaryotic and eukaryotic organisms. Several recent studies suggest important role
played by acetylation in SA-mediated signaling in plants. However, the effect of SA/ SA-mediated pathway on lysine acetylation/deacetylation is not clear. Salicylic Acid Binding Protein 2 (SABP2) is an enzyme which known to play an important role in SA mediated pathway converting the methyl salicylate (MeSA) to salicylic acid and activates Systemic Acquired Resistance (SAR). A yeast-two hybrid screening was used to identify the interaction between SABP2 and other plant proteins. Several proteins were identified as Salicylic Acid Binding Protein Interacting Proteins (SBIP). SBIP-428 is one of the interacting proteins. Bioinformatics analysis of SBIP-428 showed to SIR2 super family of proteins. SIR2 proteins are NAD+ dependent deacetylases. These proteins regulate acetylation status of proteins. Full length SBIP-428 was RT-PCR amplified and cloned using Gateway system. For biochemical characterization full length coding region of SBIP-428 was subcloned into pDEST 17. Epitope (6x His) tagged SBIP-428 was expressed in E.coli and recombinant protein purified done using Ni-NTA followed by mono-Q chromatography using AKTA protein purification system. SDS-PAGE and western analysis using monoclonal Anti-polyHistidine antibody was used to detect the SBIP-428 in every step of purification. Why SABP2 interact with SBIP-428 is an important question. Does this interaction modulate the deacetylase activity of SBIP-428? Does SA, a product of SABP2 catalytic activity modulate the deacetylase activity of SBIP-428? To detect acetylated proteins, anti-Lysine antibodies are being used for western blot analysis. To analyze the role of SABP2 on acetylation of tobacco proteins we are using transgenic tobacco plants (1-2) silenced in SABP2 expression. As a control, transgenic plants containing an empty silencing vector (C3) will be used. Another transgenic plant NahG which do not accumulate SA will also be used. This study will help to better understanding of protein interaction cascades in SA mediated plant defense. It will also show the way to establish the understanding of the role of lysine acetylation in plant which is poorly known so far.

THE RELATIONSHIP BETWEEN CALCIUM INTAKE AND HYPERTENSION AMONG OBESE ADULTS

Yang Chen1, Dr. Shimin Zheng1,2, Dr. Liang Wang1
1Department of Biostatistics and Epidemiology, College of Public Health
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN

Background: Hypertension is defined as an elevated systolic blood pressure (SBP ≥ 140 mmHg), or an
elevated diastolic blood pressure (DBP ≥ 90 mmHg). The prevalence of hypertension is high in obese
population. The potential effects of inadequate calcium intake on hypertension are receiving growing
attention. The aim of the study was to examine the association between calcium intake and hypertension among obese adults. Methods: A total of 14,856 obese adults aged 20 years or older were obtained from the 1999-2010 National Health and Nutrition Examination Survey. Analysis of variance was used to examine if there was a relationship between calcium intake and blood pressure, SBP or DBP. Multiple logistic regressions were used to examine the association between calcium intake and hypertension after adjusting for potential confounders (energy intake, age, race, education level, alcohol use, smoking, and diabetes). Results: Prevalence of hypertension decreased with an increasing quartile of calcium intake (p<0.0001). Multiple logistic regression showed that lowest quartile of calcium intake was associated with an increased risk of elevated SBP and elevated DBP (Odds Ratio (OR) =1.332, 95% Confidence Interval (CI): 1.084-1.636; OR=1.700, 95% CI: 1.234-2.342, respectively). Compared with adults in the highest quartile of calcium intake, those in lowest quartile had 1.4 times increased risk of hypertension (OR=1.400, 95% CI: 1.157-1.694). Conclusion: Our study provides support of research perspective that inadequate calcium intake may increase the risk of hypertension, high SBP, or high BDP among obese adults. Further studies are needed to understand physiological mechanism. Increasing the calcium intake in obese adults can be considered as a strategy to prevent hypertension.

HUMAN ENTEROPEPTIDASE LIGHT CHAIN: BIOENGINEERING OF RECOMBINANTS AND KINETIC INVESTIGATIONS OF STRUCTURE AND FUNCTION

Eliot T. Smith1 and David A. Johnson1,2
2Department of Biomedical Sciences, Quillen College of Medicine
2Institute for Quantitative Biology
East Tennessee State University, Johnson City, TN

The serine protease enteropeptidase exhibits a high level of substrate specificity for the cleavage sequence DDDDK~X, making this enzyme a useful tool for the separation of recombinant protein fusion domains. In an effort to improve the utility of enteropeptidase for processing fusion proteins and to better understand its structure and function, two substitution variants of human enteropeptidase, designated R96Q and Y174R were created and produced as active (>92%) enzymes secreted by Pichia pastoris with yields in excess of 1.7 mg/Liter. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 10^6 M^-1 sec^-1) and DDDDR (kcat/KM = 1.89 x 10^7 M^-1 sec^-1) relative to all other enteropeptidase variants reported to date. BPTI inhibition of Y174R was significantly decreased. Kinetic data demonstrate the important contribution of the positively charged residue 96 to extended substrate specificity in human enteropeptidase. Modeling shows the importance of the charge–charge interactions in the extended substrate binding pocket.

 

 

 

2010:
1. Research supported by NSF-TEQB
2. Research supported by Ronald McNair program
3. Research supported by NSF-REU

Student Project Description Faculty Advisor
Carly Manning 1 Occurrence of the Fecal Pathogens E.coli 0517:H7 and Salmonella
Phil Scheuerman (environmental health)
McKayla Johnson 1

The effects of UV-induced DNA damage on Cell  Growth and DNA Repair

Phil Musich (molecular biology and biochemistry)
Ava Gooding 1 Analyzing Properties of Secondary RNA structures using graph theory Trina Wooten (mathematics & statistics)
Derek Crouthers 1 Analyzing Properties of Secondary RNA structures using graph theory Trina Wooten
 (mathematics & statistics)
Rebecca Leonard 1 Analyzing Properties of Secondary RNA structures using graph theory Trina Wooten
 (mathematics & statistics)
Sharon Cameron 1 Game theory with Defender Learning Lev Yampolsky (biological sciences)
Brigette Prater-Thompson 1   Lev Yampolsky (biological sciences)
Lindsey McAmis 1 Role of Stress in the Onset of Diabetes Mellitus in Mice Edward Onyango (health sciences)
Byron Roland 1 Fire effects on forest habitats: an individual based model Istvan Karsai (biological science)
Austin Rhoten 1 Ab Initio and Semi-empirical Quantum Chemistry Vibrational Calculations of a class of Bisphosphonates Scott Kirkby (chemistry)
Lauren Brooks 1

A Profile of Rural Southern Appalachian HIV Patients: VA versus Community

Foster Levy (biological sciences)
Chelsea Ross 1 Secondary RNA structure, combinatorics and random graphs Anant Godbole
(mathematics & statistics)
Landon Zink 1,3   Yue Zou (biochemistry & molecular biology)
Cade Herron 2 Classifying Human ABC transporters using  graph descriptors Debra Knisley
(mathematics & statistics)
Mychal Bolton 1   Edward Onyango (health sciences)
Lindsey Rogerson 1   Istvan Karsai (biological science)
Haley Stinnett

Localization of calbindin-D28K in the extraembryonic membranes of a highly placentotrophic lizard, Pseudemoia pagenstecher i

James Stewart (biological sciences), Rebecca Pyles(biological sciences) and Tom Ecay (physiology)
Xinyun Cao

Toll-Like Receptor 4 (TLR4) Deficiency and Antagonist for Trauma-Hemorrhage

Race Kao (surgery)
Lee Ragsdale

Chronic Methylphenidate Treatment Decreases BDNF Concentration in the Striatum of Female Adolescent Rats

Russ Brow n (Psychology)
Jeremy Brooks 1 T1 Mapping Diffuse Myocardial Fibrosis Christopher Kramer (Univ.of Virginia)
Laura Catron Immigration Laws & Immigrant Health:   Modeling the Spread of Tuberculosis in Arizona Baojun Sung (University of Montclair)
Joseph Powell The Baa-tany Goat Project & Roan Mountain’s Unique Alder Bald Levy Foster (biological sciences)




2009
1.
Research supported by NSF-TEQB

Student Project Description Faculty Advisor
Jeremy Brooks 1 Comparing and Contrasting Different Forms of the Fitzhugh-Nagumo Model Jeff Knisley (mathematics)
Lindsey McAmis 1 Role of Stress in the Onset of Diabetes Mellitus in Mice Edward Onyango (health sciences)
Lauren Brooks 1 Effect of Diet on the Expression of the Protective Mucins in the Gastrointestinal Tract of Mice Edward Onyango (health sciences)
Sharon Cameron 1 Defender/Offender Game: With Defender Learning Lev Yampolsky (biological sciences)
Landon Zink 1 RPA and P53 Interaction Yue Zou (biochemistry & molecular biology)
Carly Manning 1 Exploration of Food Sources by Ants: An agent based simulation Istvan Karsai (biological science)


2008
1. Research supported by ETSU Student-Faculty Collaboration grant-Honors College
2. Research supported by the MAA with funding from NSF, NSA and the Moody Foundation

Student Project Description Faculty Advisor
Alison Coston 2 Graph-theoretic modeling of the family of Human ABC transporters Debra Knisley
Kenneth Lauderdale 2 Multiple sequence alignment algorithms based on numerical sequences Debra Knisley
Annette Nti 2 Network analysis of the CFTR interaction network Debra Knisley
Maribel Reyes 2 Predicting HIV-1 drug resistance due to specific amino acid substitutions Debra Knisley
DaVaughn Lauderdale 2 Invariants of line graphs from RNA Debra Knisley
Maleka Khambaty 1 Modeling habitat fragmentation  

2007

1. Research supported by
 the MAA with funding from NSF, NSA and the Moody Foundation

Student Project Description Faculty Advisor
Steve Patton 2 Descriptors derived from line graphs of RNA trees Debra Knisley
Leonard Roberts 2 Biomolecular invariants of amino acid trees Debra Knisley
Duran Williams 2 Small world properties of networks Debra Knisley

2006
 1. Research supported by ETSU Student-Faculty Collaboration grant-Honors College
Student Project Description Faculty Advisor
Daniel Lamb 1 Graph-theoretic models of the human Hsp70 ATPase domain (honors thesis) Debra Knisley
Jennifer Stepp 1 Using graph theory to develop a predictive model for OB-folds in proteins (honors thesis) Drs. Lev Yampolsky & Debra Knisley

2005
1. Research supported by NSF-UBM
2.
Research supported by  the MAA with funding from NSF, NSA and the Moody Foundation

Student Project Description Faculty Advisor
Holly Hicks 1 Graph theoretic based models of UDP-Glucosyltransferases Drs. Celia McIntosh and Debra Knisley
Brad Wild 1 Predicting function of proteins from structural information using graph theory Drs.  Celia McIntosh Debra Knisley
Daniel Lamb 1 Graph theoretic based models of UDP-Glucosyltransferases Drs.  Celia McIntosh & Debra Knisley
Shannon McConnell 1 Graph theoretic based models of UDP-Glucosyltransferases Drs.  Celia McIntosh & Debra Knisley
Mike Phillips 1 Bottom up approach of division of labor Drs. Steve Karsai and Jeff Knisley
Dmitry Yampolsky 1 Computational approach of division of labor Drs. Steve Karsai and Jeff Knisley
Patricia Carey 1 Neural networks and microarrays Drs. Karl Joplin and Jeff Knisley
Jennifer K. Cooke 1 Diapause in Sarcophaga Drs. Karl Joplin and Jeff Knisley
Erin Ashton 1 Statistical approach to multifactorial microarray analysis Drs. Edith Seier and Lev Yampolsky
Tywanna Anderson 2 Using graphical invariants to quantify secondary RNA structure Dr. Debra Knisley
Huda Hussein 2 Predicting RNA structure by domination invariants Dr. Debra Knisley
Veranda Moffet 2 On the chromatic number of DNA graphs Dr. Debra Knisley
Glenda Span 2 On the regularity of DNA graphs Dr. Debra Knisley

2004
1. Research supported by NSF-UBM
2.
Research supported by  the MAA with funding from NSF, NSA and the Moody Foundation

Student Project Description Faculty Advisor
Mike Phillips 1 Agent-based modeling of insect societies Drs. Steve Karsai and Jeff Knisley
Leanna Horton 1 Markovian and connected palindromes Dr. Anant Godbole
Patricia Carey 1 Neural networks and microarray expression data Drs. Karl Joplin and Jeff Knisley
Georganna Rose 1l Characterization of genes differentially regulated in Sarcophaga crassipalpis using neural network and microarray analysis Drs. Karl Joplin and Jeff Knisley
Emily Mullersman 1 A game theoretical model for intraspecific brood parasitism  
Jennifer Whittington 1 Game theory model of intraspecific nest parasitism Drs. Lev Yampolsky and Jeff Knisley
Brad Wild 1 Graph model of chaperone proteins Hsp70 and Hsp60 Dr. Debra Knisley
Daniel Lamb 1 Graph models of DNA repair enzymes Drs. Debra Knisley and Yue Zou
Francesca Duncan 2 Combinatorial study of siRNA Dr. Debra Knisley
Mariam Konate 2 Combinatorial study of siRNA Dr. Debra Knisley
Jeremy Smith 2 Graph-theoretic models of secondary RNA structure Dr. Debra Knisley
Ary Clemons 2 Combinatorial representations of RNA Dr. Debra Knisley
Luke Drake Using graphical parameters of trees to predict secondary RNA structures Dr. Debra Knisley


2003

Student Project Description Faculty Advisor
Katherine Williams Characterizing some graphical properties of secondary protein structures Debra Knisley