Appalachian Student Research Forum
Centre at Millennium Park • Johnson City, TN
Participant Count by Dept. - TBA
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All applicants must submit an abstract along with their registration info. An abstract is a paper used in academic research to summarize a completed study or other project. If done well, it makes the reader want to learn more about your research.
Start drafting your abstract now using a word processor, such as MS Word, so that as soon as you've submitted your registration information, you can paste your abstract text into the body of an e-mail message and send it to email@example.com . Do NOT wait until you register to compose your abstract!
Please follow the rules outlined here when developing your abstract. You may refer to the Sample Abstract at the bottom of this page as a good example.
submission deadline for registration and abstract submission is
February 26, 2010.
After you've completed drafting your abstract, you may register for the Forum:
QUANTITATIVE PCR ANALYSIS OF MOUSE TOLL-LIKE RECEPTORS
Cerrone Foster and John Laffan, Department of Microbiology, College of Medicine, East Tennessee State University, Johnson City, TN
The immune system is a complex and varied defense mechanism used to fight disease and infection. One way the body recognizes infection is through recognition of Pathogen Associated Molecular Patterns (PAMPs). Two known PAMPs, lipopolysaccharide (LPS) and glucan, are microbial products that can activate the immune system. However, the intracellular signaling pathways of the immune system are not clearly defined. It has recently been found that Toll-like receptors (TLRs) are involved in this signaling process. Stimulation of these receptors by PAMPs can initiate a signaling cascade, resulting in activation of genes needed to illicit an immune response. We therefore investigated the quantitative regulation of TLR2 and TLR4 in the presence of LPS and glucan. Using a mouse macrophage cell line (J774a.1 cells), LPS and glucan were added (1 ug/ml) to the cells or equal volume of carrier was added as a control. RNA was isolated at 1,4, and 24 hour time intervals. The RNA as reversed transcribed using a oligo dT primer and that cDNA was quantified using Quantitative PCR. Primer sets specific for TLR2 and TLR4 were designed and the reactions were run in a BioRad iCylcer real-time PCR machine. In the presence of LPS, TLR2 and TLR4 decreased during the early time intervals and dramatically increased at the 24-hour interval. In the presence of glucan, there was no significant change in TLR2 and TLR4 mRNA over time. Results of this work identified an early down regulation as well as late up regulation of TLR2 and TLR4 mRNA in the presence of LPS. This work will be a useful tool in understanding the roles of TLR2 and TLR4 in the immune response. Understanding the role of these TLRs during immune response can lead to the development of novel drugs to treat disease and infection.
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