Our overall research program related to plant secondary metabolism, specifically flavonoid
biosynthesis and regulation. We have isolated a flavonol-specific glucosyltransferase
from grapefruit which possesses unique substrate and regiospecificity. We have used
bioinformatics, sequence alignments, 3-D homology modeling, and site-directed mutagenesis
to test hypotheses related to amino acids critical for the structure and function
of this enzyme. Current efforts involve preparation of the wild type enzyme and three
mutants for protein crystallization the results of which will further test our homology
models and hypotheses.
Owens, Daniel K. and Cecilia A. McIntosh (2011) “Biosynthesis and Function of Citrus
Glycosylated Flavonoids”, Recent Advances in Phytochemistry, vol. 41:67-95 (Invited,
Daniel*, J.J., D.K. Owens, and C.A. McIntosh. (2011). Secondary Product Glucosyltransferase
Expression During Citrus paradisi Growth and Development. Phytochemistry 72:1732-1738.
Jackson*, A.R., D. Knisley, C. McIntosh, and P. Pfeiffer. (2011). Predicting Flavonoid
UGT Regioselectivity. Advances in Bioinformatics 2011( ID 506583): 15 pp. (doi:10.1155/2011/506583)
McIntosh, C.A., K. Bartoszuk, and B.J. Maxson. (2014) Integration and Synergy of
Research and Graduate Education in Science, Humanities, and Social Sciences. Critical
Conversations: An Interdisciplinary Journal 1:70-92. (invited, peer-reviewed)
McIntosh, Cecilia A. and Daniel K. Owens. (2016). Advances in Flavonoid Glycosyltransferase
Research: Integrating Recent Findings with Long-Term Citrus Studies. Phytochemistry
Reviews 15:1075-1091. doi 10.1007/s11101-016-9460-6
Devah, S.K., D.K. Owens, M.B. Sibhatu*, T. Roy Sarkar*, C.L. Strong*, V.K.P.S. Mallampalli*,
J. Asiago*, J. Cooke*, S. Kiser*, Z. Lin*, A. Wamucho*, D. Hayford*, B.E. Williams*,
P. Loftis*, M. Berhow, L.M. Pike. and C.A. McIntosh. (2016). Isolation, Recombinant
Expression and Biochemical Analysis of Putative Secondary Product Glucosyltransferases
from Citrus paradisi. J. Agric. Food. Chem. 64:1957-1969 (doi 10.1021/acs.jafc.5b05430)
Devaiah, S.P., B.M. Tolliver*, C. Zhang*, D.K. Owens, and C.A. McIntosh. (2017). Effect
of Amino Acid Insertion and Other Mutations on the Substrate and Regiospecificity
of a Citrus paradisi Flavonol-Specific 3-O-Glucosyltransferase. J Plant Biochem and
Biotech. IN PRESS DOI: 10.1007/s13562-017-0411-0
RoySarkar, T., C.L. Strong, M.B. Sibhatu, L.M. Pike, and C.A. McIntosh. 2007. Cloning,
Expression, and Characterization of a Putative Flavonoid Glucosyltransferase from
Grapefruit (Citrus paradisi) Leaves. Chapter 18 in “Concepts in Plant Metabolomics”, B.J. Nikolau and E.S. Wurtele,
eds. Springer Publishing, The Netherlands. pp. 247-259 (Invited submission).
Knisley, D., E. Seier, D. Lamb. D.K. Owens, and C.A. McIntosh. 2009. A Graph-theoretic Model
Based on Primary and Predicted Secondary Structures Reveals Functional Specificity
in a set of Plant Secondary Product UDP-Glucosyl-transferases. Proceedings of the
2009 International Conference on Bioinformatics, Computational Biology, Genomics,
and Chemoinformatics, W. Logong, M. Doble, Z. Sun, and J. Malone (eds), pp 65-72.
Owens, D.K. and C.A. McIntosh. 2009. Identification, recombinant expression, and Biochemical
Characterization of a Flavonol 3-O-Glucosyltransferase Clone from Citrus paradisi. Phytochemistry, 70:1382-1391.
Daniel, J.J., D.K. Owens, and C.A. McIntosh. 2011. Secondary product glucosyltransferase
expression during Citrus paradisi growth and development. Phytochemistry, 72:1732-1738.
Jackson, A.R., D. Knisley, C. McIntosh, and P. Pfeiffer. 2011. Predicting flavonoid
UGT regioselectivity. Advances in Bioinformatics 2011( ID 506583):15 pp. (doi:10.1155/2011/506583).
Owens, D.K. and C.A. McIntosh. 2011. Biosynthesis and function of Citrus glycosylated flavonoids, Recent Advances in Phytochemistry, 41:67-95.
Teaching Pedagogy for the Graduate Teaching Assistant (GRAD5110)