Multimode Microplate Reader

Modulus Multimode Microplate Reader

User-friendly operation – touch screen control, data download onto USB stick         MMReader

Microplate format - Accepts 96-well plates conforming to the SBS plate standard

3 Detection Modes: Luminescence, Fluorescence, Visible Absorbance

Compatible with most commercially available kits

 

 

 

  Luminescence Module:


- >8 Logs Dynamic Range
- Dual-masking System Reduces Crosstalk
- Very high sensitivity

 

 Firefly

         
The Luminescence module is separated from other measurement technologies and positioned directly above the sample well, maximizing light capture for increased sensitivity, with a detection limit of 3 x 10-21 moles Luciferase Detection or 1 x 10-18 moles ATP. The head-on photomultiplier tube has a spectral range of 350-650 nm and automatically adjusts for the optimum reading of bright or dim samples.

Dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 µl in 5µl increments.

 

 Fluorescence Module

- Epifluorescent Detection
- Multiple Optical Kits Available
- High Sensitivity

                flor. DNA

 
The Fluorescence module uses powerful wave-length matched, light-emitting PIN photodiodes (LEDs) as excitation sources. The narrow excitation/emission spectrum increases sensitivity and reduces non-specific light leakage. The fluorescence capability has a maximum linear dynamic range of 6 logs and a detection limit of 0.5 fmol/200 µl or 1 ppt of fluorescein in a 96-well plate, or 30 pg/well dsDNA with a DNA quantification dye

 

Fluorescence Module Optical Kit Application Chart
Optical Kit Typical Applications
 UV Optical Kit
Ex: 365 nm
Em: 410 - 460 nm 		 DNA Quantification (Hoechst dye)
Enzyme Activity (4-methyl-umbelliferone)
 Blue Optical Kit
Ex: 490 nm
Em: 510 - 570 nm

DNA, RNA & Protein Quantitation
 Protein Labeling (Fluorescein)
Gene Expression (EGFP, rAcGFP)

Green Optical Kit
Ex: 525 nm
Em: 580 - 640 nm		 Nucleotide Labeling
Protein Labeling (Rhodamine, Cy3)
Enzyme Activity (Rhodamine)
 Red Optical Kit
Ex: 625 nm
Em: 660 - 720 nm		 Nucleotide or Protein Labeling (Cy5)
RNA Quantitation
 AFC Optical Kit
Ex: 405 nm
Em: 495 - 505 nm		 Cell Viability Assays
Cytotoxicity Assays
Apoposis Multiplex Assays


 

 
Absorbance Module

- Flexible Filter System
- 0-5.0 OD Reading Range
- Single or Dual Wavelengths 		             Color Assay



The Absorbance module uses a large area photodiode detector to provide highly sensitive measurements covering a wide dynamic range with an accuracy that deviates less than 2%. The channel consists of a white LED with a spectral range of 450-750 nm.

 
Absorbance Module Filter Application Chart
Wavelength Typical Applications
450 ELISA, QuantiCleave™ Protease Assay
560 BCA™ Protein Assays
600 Bradford, Coomassie Blue Protein Assays
PeroXOquant™ Quantitative Peroxide Assay
750 Lowry Protein Assay

 


Fees and Requirements:

To use the Microplate readers:

  • You must be trained by a member of the MBCF staff
        
  • To schedule training: Contact us at (423) 439-8096 or via email at mbcf@etsu.edu.

Equipment use fee:

  • Modulus Microplate Reader  $7/run
         
  • SpectraMax 190 Plate Reader $7/run

 

SpectraMax 190 Microplate ReaderSpectraMax 190

The SpectraMax 190 UV-V is Microplate Spectrophotometer from Molecular Devices which enables data aquisition in endpoint, kinetic, and spectral scan modes. The multi-channel design truly mimics a dual-beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the optic fibers. The system consists of eight sample beams and detectors and eight reference beams and detectors to deliver both superior precision and speed-of-reading across the microplate through 4.0 OD.

  • Microplate Format- Accepts 96-well plates conforming to the SBS plate standard
        
  • Shaking feature allows in-plate mixing
         
  • The SpectraMax 190 uses SoftMax Pro Microplate Data Aquisition and Analysis Software.
        
  • Up to 6 different wavelengths can be measured during one reading.
        
  • The patented PathCheck sensor senses the depth of the liquid in each microplate well and normalizes the absorbance value to a 1 cm pathlength.
        
  • The temperature control feature allows incubation at 37°C for live cell assays
             .
  • Temperature Specifications:
        
    •  Temperature range: Ambient +4– 45°C
          
    •  Temperature uniformity (microplate): ±0.5°C at 37°C, well-to-well
          
    •  Temperature stabilization time: 30 minutes maximum upon initiation
          
    • Reading Chambers: Isothermal when temperature regulation not enabled, <1°C 

 

  • Photometric Performance Specifications
                   

    • Wavelength range: 190–850 nm

    • Wavelength selection: Monochromator, tunable in 1 nm increments
          
    • Wavelength bandwidth: 2 nm 
          
    • Wavelength accuracy: < ±2.0 nm
         
    • Wavelength repeatability: ±0.2 nm
          
    • Photometric range: 0–4.0 OD
          
    • Photometric resolution: 0.001 OD
          
    • Photometric linearity (405 nm): 0–3.000 OD
          
    • Photometric accuracy (190-850 nm): < ±0.006 OD ±1.0%, 0–2.0 OD
             

    • Photometric precision (190–850 nm): < ±0.003 OD ±1.0%, 0–2.0 OD