Data Acquisition and Imaging
Molecular Biology Core Facility Data Acquisition Services
Your core has many options available for data acquisition using our multimode plate reader or digital darkroom for collection of absorbance, fluorescent, or luminescent signal.
Modulus Multimode Plate Reader
User-friendly microplate reader with colorimetric, fluorescent, and luminescent capabilities. Touch screen operation with data download to a USB drive. Accepts all 96-well plates conforming to the SBS plate standard.
The Absorbance module uses a large area photodiode detector to provide highly sensitive measurements covering a wide dynamic range with an accuracy that deviates less than 2%. The channel consists of a white LED with a spectral range of 450-750 nm.
- Flexible Filter System
0-5.0 OD Reading Range
Single or Dual Wavelengths
Absorbance Mode Filter Applications
Wavelenth Typical Applications 450 ELISA, QuantiCleave Protease Assay 560 BCA Protein Assays 600 Bradford and Coomassie Blue Protein Assays, PeroXOquant Quantitative Peroxide Assay 750 Lowry Protein Assay
The Fluorescence module uses powerful wave-length matched, light-emitting PIN photodiodes (LEDs) as excitation sources. The narrow excitation/emission spectrum increases sensitivity and reduces non-specific light leakage. The fluorescence capability has a maximum linear dynamic range of 6 logs and a detection limit of 0.5 fmol/200 l or 1 ppt of fluorescein in a 96-well plate, or 30 pg/well dsDNA with a DNA quantification dye.
- Epifluorescent Detection
Multiple Optical Kits Available
Fluorescence Mode Optical Kit Applications
UV Optical Kit
Ex: 365 nm
Enzyme Activity (4-methyl-umbelliferone)
Blue Optical Kit
Ex: 490 nm
Em: 510-570 nm
DNA, RNA, and Protein Quantification
Protein Labeling (Florescein)
Gene Expression (EGFP, rAcGFP)
Green Optical Kit
Ex: 525 nm
Em: 580-640 nm
Protein Labeling (Rhodamine, Cy3)
Enzyme Activity (Rhodamine)
Red Optical Kit
Ex: 625 nm
Nucleotide or Protein Labeling (Cy5)
AFC Optical Kit
Ex: 405 nm
Em: 495-505 nm
Cell Viability Assays
Apoptosis Multiplex Assays
The Luminescence module is separated from other measurement technologies and positioned directly above the sample well, maximizing light capture for increased sensitivity, with a detection limit of 3 x 10-21 moles Luciferase Detection or 1 x 10-18 moles ATP. The head-on photomultiplier tube has a spectral range of 350-650 nm and automatically adjusts for the optimum reading of bright or dim samples.
Dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 ul in 5 ul increments.
- 8 Logs Dynamic Range
Dual-masking System Reduces Crosstalk
Very high sensitivity
Syngene G:Box XX6
The SYNGENE G:BOX iChemi XT is a high performing system suitable for fluorescence and chemiluminescence using a 6 megapixel camera. It has a large aperture lens making it perfect for low light level applications where longer exposures are needed.