Sample Quantitation and Qualitation
Molecular Biology Core Facility Sample Quantitation and Qualitation Services
Sample quantity and quality are the greatest determinants of a successful molecular experiment. Your core houses instrumentation for several complementary methods to ensure that you are using the best samples for your downstream applications.
The NanoDrop One spectrophotometer accurately quantifies DNA, RNA, or protein from as little as one microliter of sample. Visualization of the absorbance spectra and onboard Acclaro Sample Intelligence technology makes it easy to identify potential contaminants.
The NanoDrop One can also
- Measure microarray or labeled proteins- quantify nucleic acids or proteins labeled with up to two fluorescent dyes (detection of dye concentration as low as 0.2 pM/mL)
Protein quantitation by A280, A205, BCA, Bradford, Lowry, or Pierce 660
Monitor growth rate of microbial cell cultures by OD600
Use custom methods for anything spectrally compatible
The Qubit Fluorometer is our most specific quantification instrument.
Use the Qubit for
- dsDNA, ssDNA, and RNA quantitation in 2 minutes
Protein quantitation in 15 minutes
MyQubit assays for anything spectrally compatible, including: mmmmm m microRNA, c, cholesterol, ggggggggggalactose, glucose, glutamic acid, peroxide, and sucrose
Agilent 2100 Bioanalyzer and 2200 Tapestation
The Agilent™ 2100 Bioanalyzer and the Agilent™ 2200 Tapestation are high-throughput microfluidics-based platforms for rapid analysis of DNA, RNA, and Protein.
The Bioanalyzer RNA kits allow dependable and precise integrity checks and sample quantitation prior to any RNA-dependent application. This ensures the data from downstream research processes, such as gene expression microarray, qPCR, and transcriptome sequencing, are valid and reliable.
Besides sample quality control of next-generation sequencing (NGS) libraries, additional research applications for the Bioanalyzer DNA assays include PCR amplicon analysis, multiplex PCR amplicons, gene expression analysis by RT-PCR, restriction fragment analysis, and the detection of targeted cleavage in gene editing studies.