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Molecular Biology Core Facility

Quillen College of Medicine

Next-Gen Sequencing

ILLUMINA MISEQ                                                                      Better MiSeq picture

The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.



Workflow MiSeq



Cluster Generation and Sequencing:

MiSeq Reagent Kit v2

 Read length Total Time* Output
 1 x 36 bp  4 hours  540-610 Mb
 2 x 25 bp 5.5 hours   750-850 Mb
 2 x 150 bp  24 hours  4.5-5.1 Gb
 2 x 250 bp  39 hours  7.5-8.5 Gb
Reads Passing Filter
Single Reads 12-15
Paired-End Reads 24-30
Quality Scores
1 x 36 bp >90% bases higher than Q30
2 x 25 bp >90% bases higher than Q30
2 x 150 bp >80% bases higher than Q30
2 x 250 bp >75% bases higher than Q30


MiSeq Reagent Kit v3

Read Length Total Time* Output
2 x 75 bp 24 hours 3.3-3.8 Gb
2 x 300 bp 65 hours 13.2-15 Gb
Reads Passing Filter
Single Reads 22-25 M
Paired-End Reads 44-50 M



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