Next-Gen Sequencing
CONTACT THE FACILITY PRIOR TO BEGINNING YOUR NGS PROJECT. WE CAN DIRECT YOU IN APPROPRIATE CHOICES FOR YOUR APPLICATION.
- MBCF CAN ASSIST YOU IN PREPARING YOUR LIBRARIES
- UTILIZE OUTSIDE VENDORS FOR PROCESSING
- YOU CAN ANAYLYZE YOUR DATA USING QIAGEN CLC GENOMICS WORKBENCH (housed in the MBCF)
ILLUMINA MISEQ
The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.
TYPICAL WORK FLOW
SPECIFICATIONS:
Cluster Generation and Sequencing:
MiSeq Reagent Kit v2
Read length | Total Time* | Output |
1 x 36 bp | 4 hours | 540-610 Mb |
2 x 25 bp | 5.5 hours | 750-850 Mb |
24 hours | 4.5-5.1 Gb | |
2 x 250 bp | 39 hours | 7.5-8.5 Gb |
Reads Passing Filter | |
Single Reads | 12-15 |
Paired-End Reads | 24-30 |
Quality Scores | |
1 x 36 bp | >90% bases higher than Q30 |
2 x 25 bp | >90% bases higher than Q30 |
2 x 150 bp | >80% bases higher than Q30 |
2 x 250 bp | >75% bases higher than Q30 |
MiSeq Reagent Kit v3
Read Length | Total Time* | Output |
2 x 75 bp | 24 hours | 3.3-3.8 Gb |
2 x 300 bp | 65 hours | 13.2-15 Gb |
Reads Passing Filter | |
Single Reads | 22-25 M |
Paired-End Reads | 44-50 M |