Overview
The Olympus BX63 microscope supports brightfield and fluorescence imaging for research applications. Select a section below to learn more about system capabilities, training and usage.
Olympus BX63 Microscope Information
The Olympus BX63 is an upright microscope that produces high-resolution brightfield and fluorescence images of properly fixed and mounted, histologically stained or fluorescently labeled tissues and cells.
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Key features include:
- Fully motorized system for precise control
- Fixed stage design for improved stability
- Motorized Z-drive for accurate focusing
- Support for multidimensional imaging and Z-stack acquisition
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Imaging Capabilities
The BX63 supports advanced imaging through:
- Motorized nosepiece for precise focusing
- Ultrasonic piezo-driven stage for smooth and accurate movement
- Controller pad with selectable guidance mode and full mode
- High-sensitivity imaging for fluorescence applications
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Fluorescence Technology
The system includes optimized components for fluorescence imaging:
- Interchangeable fluorescence mirror units
- High-transmission coatings for improved signal detection
- Reduced stray light for increased sensitivity
- Sharp color separation for fluorescence imaging
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Objectives and Optics
The system uses UIS2 objectives with high numerical aperture and reduced autofluorescence.
Available objectives:
- 2x PlanApo (0.08 NA)
- 4x UPlanXApo (0.16 NA)
- 10x UPlanXApo (0.40 NA)
- 20x UPlanXApo (0.80 NA)
- 40x UPlanXApo (0.95 NA)
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Cameras and Imaging Software
Images are captured using:
- Hamamatsu ORCA-Flash 4.0 LT camera for fluorescence imaging
- Olympus DP74 camera for brightfield imaging
The system is operated using Olympus cellSens software, which provides:
- Customizable interface and controls
- Automated image acquisition
- Advanced image analysis tools
cellSens Dimension supports automated imaging and data processing.
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Training Requirements
Training is provided by the core manager.
All users must complete training before using the microscope independently.
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Reservations and Access
After completing training, users may coordinate with the core manager to schedule time on the microscope.
Users are encouraged to:
- Plan usage in advance
- Limit sessions to half-day increments when demand is high
- Communicate scheduling needs with the core
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Usage Fees
There are no fees associated with using the Olympus BX63 microscope.
All ETSU students and staff may use the system after completing training.
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Policies and Procedures
All users must follow these guidelines:
- Bring a personal storage device for saving data
- Data on workstation computers may be periodically removed
- Long-term data storage is not guaranteed
- No food or drink is allowed in the microscopy room
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Publication Acknowledgement
Users are expected to acknowledge the Microscopy Core Facility in manuscripts and publications when data obtained from the facility is used.